Rapid expansion of ideas using engineered nanoparticles to achieve various agriculture-relevant functions (agri-NPs) over the past decade, has been revamping the available tools for studying these NPs in soil, plants, and irrigation-outflow (agri-matrices). The development of high-throughput analytical tools is imperative for defining the future scale of agri-NP applications. Here we summarized the available methods for studying the behavior and ecotoxicity of agri-NPs in relevant matrices, aiming to provide a comprehensive coverage of the latest progress in this field and to identify future-research urgencies. To render the readers with a general background of agri-NPs, we first introduced agri-NPs’ various designated functions, materials, targets, and efficacy/environmental uncertainties. For future work, we propose to prioritize upgrading of existing in-situ tools for more diverse analytical capabilities, further development of higher-throughput screening methods that may enable automated data collection/analysis, development of more non-target analytical protocols, and extending the latest methods in biomedical-NP detection/quantification to study agri-NPs in plants.

在过去十年中，使用工程纳米粒子实现各种农业相关功能 (agri-NPs) 的想法迅速扩展，一直在改进用于研究土壤、植物和灌溉流出物 (agri-matrices) 中这些 NPs 的可用工具。开发高通量分析工具对于定义农业 NP 应用的未来规模势在必行。在这里，我们总结了研究相关基质中农业 NPs 的行为和生态毒性的可用方法，旨在全面覆盖该领域的最新进展并确定未来研究的紧迫性。为了让读者了解 agri-NPs 的一般背景，我们首先介绍了 agri-NPs 的各种指定功能、材料、目标和功效/环境不确定性。为了以后的工作，用于更多样化分析能力的原位工具，进一步开发可实现自动化数据收集/分析的更高通量筛选方法，开发更多非目标分析协议，并将生物医学-NP 检测/量化的最新方法扩展到研究植物中的 agri-NPs。