Measurement of cell surface coverage has become a common technique for the assessment of growth behavior of cells. As an indirect measurement method, this can be accomplished by monitoring changes in electrode impedance, which constitutes the basis of electric cell-substrate impedance sensing (ECIS). ECIS typically yields growth curves where impedance is plotted against time, and changes in single cell growth behavior or cell proliferation can be displayed without significantly impacting cell physiology. To provide better comparability of ECIS curves in different experimental settings, we developed a large toolset of R scripts for their transformation and quantification. They allow importing growth curves generated by ECIS systems, edit, transform, graph and analyze them while delivering quantitative data extracted from reference points on the curve. Quantification is implemented through three different curve fit algorithms (smoothing spline, logistic model, segmented regression). From the obtained models, curve reference points such as the first derivative maximum, segmentation knots and area under the curve are then extracted. The scripts were tested for general applicability in real-life cell culture experiments on partly anonymized cell lines, a calibration setup with a cell dilution series of impedance versus seeded cell number and finally IPEC-J2 cells treated with 1% and 5% ethanol.

中文翻译：

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电池-基板阻抗传感生长曲线的建模和微分量化

细胞表面覆盖的测量已成为评估细胞生长行为的常用技术。作为一种间接测量方法，这可以通过监测电极阻抗的变化来实现，这构成了电池 - 基板阻抗传感 (ECIS) 的基础。ECIS 通常会生成生长曲线，其中阻抗与时间作图，并且可以显示单细胞生长行为或细胞增殖的变化，而不会显着影响细胞生理学。为了在不同的实验设置中提供更好的 ECIS 曲线可比性，我们开发了一个大型 R 脚本工具集用于它们的转换和量化。它们允许导入由 ECIS 系统生成的增长曲线、编辑、转换、绘制并分析它们，同时提供从曲线上的参考点提取的定量数据。量化是通过三种不同的曲线拟合算法（平滑样条、逻辑模型、分段回归）实现的。从获得的模型中，然后提取曲线参考点，例如一阶导数最大值、分割节点和曲线下面积。这些脚本在部分匿名细胞系的真实细胞培养实验中的普遍适用性、具有阻抗与接种细胞数的细胞稀释系列的校准设置以及最后用 1% 和 5% 乙醇处理的 IPEC-J2 细胞进行了测试。然后提取曲线参考点，例如一阶导数最大值、分割节点和曲线下面积。这些脚本在部分匿名细胞系的真实细胞培养实验中的普遍适用性、具有阻抗与接种细胞数的细胞稀释系列的校准设置以及最后用 1% 和 5% 乙醇处理的 IPEC-J2 细胞进行了测试。然后提取曲线参考点，例如一阶导数最大值、分割节点和曲线下面积。这些脚本在部分匿名细胞系的真实细胞培养实验中的普遍适用性、具有阻抗与接种细胞数的细胞稀释系列的校准设置以及最后用 1% 和 5% 乙醇处理的 IPEC-J2 细胞进行了测试。