In our study, the dietary effect of dandelion flower (DF) extract on growth performance, hemto-biochemical parameters, and transcriptomic responses of growth and antioxidant genes in rainbow trout were investigated. Accordingly, three hundred fingerlings were randomly stocked into 15 tanks and fed with different levels of DF extract (0 [control], 1, 2, 3, and 4 g/kg) for 8 weeks. The results showed the weight gain was significantly increased in 3 g/kg DF extract-supplemented group. The feed conversion ratio was significantly decreased in 3 and 4 g/kg DF extract treatments compared to the control group. The highest number of red blood cells and hematocrit level were observed in 3 g/kg DF extract treatment compared to 1 g/kg DF extract and the control group. A significant rise in the serum high-density lipoprotein, glutathione peroxidase (GPx), and catalase (CAT) was obtained in 3 g/kg DF extract. However, the levels of serum alanine and aspartate amino transaminase, and glucose were remarkably decreased at 3 g/kg DF added-group. The intestinal protease activity was significantly enhanced in the fish fed 3 g/kg DF extract compared to others. The body crude protein was also remarkably increased in the fish fed 3 g/kg DF extract. The transcriptomic responses of growth hormone, insulin-like growth factor-1, and ghrelin genes were notably up-regulated in the treated fish, especially in 3 g/kg DF extract. However, the expression of antioxidant-related genes (SOD, CAT, and GPx) was significantly decreased in DF extract-added groups. Overall, the optimal inclusion of DF extract in rainbow trout diet was ranged from 2.83 to 3.13 g/kg and 2.59 to 2.75 g/kg to improve growth performance and antioxidant status, respectively.

在我们的研究中，研究了蒲公英花 (DF) 提取物对虹鳟鱼生长性能、血液生化参数以及生长和抗氧化基因转录组反应的饮食影响。因此，将 300 条鱼种随机放入 15 个鱼缸中，并用不同水平的 DF 提取物（0 [对照]、1、2、3 和 4 g/kg）饲喂 8 周。结果表明，3 g/kg DF 提取物补充组的体重增加显着增加。与对照组相比，3 和 4 g/kg DF 提取物处理的饲料转化率显着降低。与 1 g/kg DF 提取物和对照组相比，在 3 g/kg DF 提取物处理中观察到的红细胞数量和血细胞比容水平最高。血清高密度脂蛋白谷胱甘肽过氧化物酶（GPx）显着升高，在 3 g/kg DF 提取物中获得过氧化氢酶 (CAT)。然而，血清丙氨酸、天冬氨酸氨基转氨酶和葡萄糖水平在 3 g/kg DF 添加组显着降低。与其他鱼相比，喂食 3 g/kg DF 提取物的鱼的肠道蛋白酶活性显着增强。饲喂3 g/kg DF提取物的鱼的身体粗蛋白也显着增加。生长激素、胰岛素样生长因子-1 和 ghrelin 基因的转录组反应在处理过的鱼中显着上调，尤其是在 3 g/kg DF 提取物中。然而，抗氧化相关基因的表达（与其他鱼相比，喂食 3 g/kg DF 提取物的鱼的肠道蛋白酶活性显着增强。饲喂3 g/kg DF提取物的鱼的身体粗蛋白也显着增加。生长激素、胰岛素样生长因子-1 和 ghrelin 基因的转录组反应在处理过的鱼中显着上调，尤其是在 3 g/kg DF 提取物中。然而，抗氧化相关基因的表达（与其他鱼相比，喂食 3 g/kg DF 提取物的鱼的肠道蛋白酶活性显着增强。饲喂3 g/kg DF提取物的鱼的身体粗蛋白也显着增加。生长激素、胰岛素样生长因子-1 和 ghrelin 基因的转录组反应在处理过的鱼中显着上调，尤其是在 3 g/kg DF 提取物中。然而，抗氧化相关基因的表达（SOD、CAT和GPx ) 在添加 DF 提取物的组中显着降低。总体而言，虹鳟鱼饲料中 DF 提取物的最佳添加量为 2.83 至 3.13 g/kg 和 2.59 至 2.75 g/kg，以分别提高生长性能和抗氧化状态。