当前位置: X-MOL 学术Aquaculture › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular cloning of IgT, IgD and analysis the IgH locus in largemouth bass (Micropterus salmoides) and immune response upon bacterial infection
Aquaculture ( IF 3.9 ) Pub Date : 2021-08-05 , DOI: 10.1016/j.aquaculture.2021.737291
Guangkun Han 1, 2 , Xue Zhai 1 , Zhengben Wu 1 , Hui Jiang 1 , Sha Wu 1 , Yang He 3 , Chuanjie Qin 3 , Zhen Xu 2
Affiliation  

Immunoglobulins (Igs) are crucial components of the immune response in vertebrates including teleost fish. In the present study, we firstly cloned the full-length cDNA of IgT and IgD heavy chain genes in largemouth bass (Micropterus salmoides), one of the important farmed fish species in China. Importantly, IgH locus covering τ, μ and δ genes of largemouth bass was also illustrated. The genomic organization of the IgH locus presented as V26-D3-J5-Cτ-D6-J6-Cμ-Cδ, which was similar with some other teleost fish. Furthermore, The IgH locus of largemouth bass contained 26 VH segments and they belonged to three subgroups, whose members showed a greater than 80% amino acid identity. This founding would allow us to gain further insight into the evolution of adaptive immune system. To further understanding the immune response of immunoglobulins, we successfully constructed the infection model with bacteria (GFP–Flavobacterium columnare G4). Our results showed the expression of IgM was up-regulated in head kidney, and the expression of IgT was significantly induced mainly in skin and gill, suggesting their potential roles in systemic and mucosal immune response to bacterial infection, respectively.

更新日期:2021-08-10
down
wechat
bug