Cerebellar Purkinje neurons help compute absolute subsecond timing, but how their firing is affected during repetitive sensory stimulation with consistent subsecond intervals remains unaddressed. Here, we investigated how simple and complex spikes of Purkinje cells change during regular application of air puffs (3.3 Hz for ~4 min) to the whisker pad of awake, head-fixed female mice. Complex spike responses fell into two categories: those in which firing rates increased (at ~50 ms) and then fell (complex spike elevated "CxSE" cells), and those in which firing rates decreased (at ~70 ms) and then rose (complex spike reduced "CxSR" cells). Both groups had indistinguishable rates of basal complex (~1.7 Hz) and simple (~75 Hz) spikes, and initially responded to puffs with a well-timed sensory response of a short-latency (~15 ms), transient (4 ms) suppression of simple spikes. CxSE more than CxSR cells, however, also showed a longer-latency increase in simple spike rate, previously shown to reflect motor command signals. With repeated puffs, basal simple spike rates dropped greatly in CxSR but not CxSE cells; complex spike rates remained constant, but their temporal precision rose in CxSR cells and fell in CxSE cells. Also over time, transient simple spike suppression gradually disappeared in CxSE cells, suggesting habituation, but remained stable in CxSR cells, suggesting reliable transmission of sensory stimuli. During stimulus omissions, both categories of cells showed complex spike suppression with different latencies. The data indicate two modes by which Purkinje cells transmit regular repetitive stimuli, distinguishable by their climbing fiber signals.

中文翻译：

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小鼠小脑浦肯野神经元对常规训练和体感刺激遗漏的简单和复杂尖峰反应

小脑浦肯野神经元有助于计算绝对亚秒时间，但在以一致的亚秒间隔重复感觉刺激期间它们的放电如何受到影响仍未得到解决。在这里，我们研究了在定期向清醒、头部固定的雌性小鼠的胡须垫吹气（3.3 Hz，约 4 分钟）期间，浦肯野细胞的简单和复杂尖峰如何变化。复杂的尖峰反应分为两类：放电率增加（约 50 毫秒）然后下降（复杂尖峰升高的“CxSE”细胞），以及放电率降低（约 70 毫秒）然后上升（复合穗减少了“CxSR”细胞）。两组的基础复合物（~1.7 Hz）和简单（~75 Hz）尖峰的速率无法区分，并且最初以短延迟（~15 ms）的适时感官反应对抽吸做出反应，简单尖峰的瞬态（4 ms）抑制。然而，CxSE 比 CxSR 细胞还显示出简单尖峰率的更长延迟增加，之前显示它反映了运动命令信号。随着反复抽吸，CxSR 细胞的基础简单峰值率大大下降，但 CxSE 细胞没有；复杂的尖峰率保持不变，但它们的时间精度在 CxSR 细胞中上升，在 CxSE 细胞中下降。同样随着时间的推移，短暂的简单尖峰抑制在 CxSE 细胞中逐渐消失，表明习惯化，但在 CxSR 细胞中保持稳定，表明感觉刺激的可靠传递。在刺激遗漏期间，两类细胞都表现出具有不同潜伏期的复杂尖峰抑制。数据表明浦肯野细胞传输有规律的重复刺激的两种模式，可通过它们的攀爬纤维信号来区分。