Macrophages have been confirmed to play a significant role in the behavior of human lymphomas, albeit no consistent data are so far available in canine lymphomas. The present study characterizes the macrophages present in cases of canine nodal lymphoma and their relationship with the histological grade and the immunophenotype. Samples from the lymph nodes of 25 dogs diagnosed with lymphoma were selected. Immunohistochemistry was used to determine the tumor immunophenotype (CD3 and CD20 antibodies) and macrophage characterization (Iba1, MAC387, CD204, CD163 and iNOS antibodies). Macrophage counting was performed in 10 randomly selected, high-power fields per sample. Generalized linear models with Poisson distribution were used for statistical analysis. A significantly greater number of macrophages (Iba1+) were detected in high-grade and B-cell lymphomas. The highest amount of both M1 (iNOS+) and M2 (CD204+ and CD163+) subtypes were observed in B-cell lymphomas. High-grade lymphomas showed a greater number of CD204+ and CD163+ cells and recently recruited MAC387+ macrophages. The latter were most abundant in T than in B-cell lymphomas. In conclusion, a significant population of macrophages is present in canine lymphomas, which constitute a heterogeneous population that shows variations in the amount and immunohistochemical profile according to the histological grade and immunophenotype.

中文翻译：

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犬淋巴瘤中肿瘤相关巨噬细胞的免疫组织化学特征

巨噬细胞已被证实在人类淋巴瘤的行为中发挥重要作用，尽管迄今为止在犬淋巴瘤中没有一致的数据可用。本研究表征了犬淋巴结淋巴瘤病例中存在的巨噬细胞及其与组织学分级和免疫表型的关系。选择了 25 只诊断为淋巴瘤的狗的淋巴结样本。免疫组织化学用于确定肿瘤免疫表型（CD3 和 CD20 抗体）和巨噬细胞特征（Iba1、MAC387、CD204、CD163 和 iNOS 抗体）。在每个样本的 10 个随机选择的高倍视野中进行巨噬细胞计数。具有泊松分布的广义线性模型用于统计分析。在高级别淋巴瘤和 B 细胞淋巴瘤中检测到显着更多的巨噬细胞 (Iba1+)。在 B 细胞淋巴瘤中观察到最高数量的 M1 (iNOS+) 和 M2 (CD204+ 和 CD163+) 亚型。高级别淋巴瘤显示出更多数量的 CD204+ 和 CD163+ 细胞，并且最近招募了 MAC387+ 巨噬细胞。后者在 T 中的含量高于 B 细胞淋巴瘤。总之，犬淋巴瘤中存在大量巨噬细胞，它们构成了异质性群体，根据组织学分级和免疫表型，其数量和免疫组织化学特征显示出差异。高级别淋巴瘤显示出更多数量的 CD204+ 和 CD163+ 细胞，并且最近招募了 MAC387+ 巨噬细胞。后者在 T 中的含量高于 B 细胞淋巴瘤。总之，犬淋巴瘤中存在大量巨噬细胞，它们构成了异质性群体，根据组织学分级和免疫表型，其数量和免疫组织化学特征显示出差异。高级别淋巴瘤显示出更多数量的 CD204+ 和 CD163+ 细胞，并且最近招募了 MAC387+ 巨噬细胞。后者在 T 中的含量高于 B 细胞淋巴瘤。总之，犬淋巴瘤中存在大量巨噬细胞，它们构成了异质性群体，根据组织学分级和免疫表型，其数量和免疫组织化学特征显示出差异。