Abstract

In the current study, a new Cu(II)-containing coordination polymer (CP) with the chemical formula of {[Cu₂(H₂O)₅(tpt)(L)]·6H₂O}_n (1, tpt = 2,4,6‐tris(4‐pyridyl)‐1,3,5‐triazine and L = 3,3′‐disulfonyl‐4,4′‐biphenyldicarboxylate) has been successfully produced via a hydrothermal reaction of Cu(NO₃)₂·3H₂O with the carboxylate ligand L in the presence of the auxiliary N-donor ligand tpt. The anti-cancer activity of the new compound on retinoblastoma was evaluated and the related mechanism was explored at the same time. First of all, the Cell Counting Kit-8 (CCK-8) kit was performed to determine the viability of the Y79 retinoblastoma cells after compound treatment. Next, after compound exposure, the activation of the JAK-STA signaling pathway in the Y79 retinoblastoma cells was measured with real time reverse transcription-polymerase chain reaction (RT-PCR) assay.

摘要

在目前的研究中，一种新的含Cu(II)的配位聚合物(CP)的化学式为{[Cu ₂ (H ₂ O) ₅ (tpt)(L)] · 6H ₂ O} _n ( 1 , tpt = 2,4,6-tris(4-pyridyl)-1,3,5-triazine and L = 3,3'-disulfonyl-4,4'-biphenyldicarboxylate) 通过 Cu(NO) 的水热反应成功制备₃ ) ₂ ·3H ₂在辅助 N 供体配体 tpt 存在下，O 与羧酸盐配体 L。评价了新化合物对视网膜母细胞瘤的抗癌活性，同时探讨了相关机制。首先，进行细胞计数试剂盒8（CCK-8）试剂盒以确定Y79视网膜母细胞瘤细胞在复合处理后的活力。接下来，在化合物暴露后，用实时逆转录聚合酶链反应 (RT-PCR) 测定法测量 Y79 视网膜母细胞瘤细胞中 JAK-STA 信号通路的激活。