Imaging and Identifying BSA Biofouling through the Combination of Fluorescence Microscopy and Flux Experiments,Clean - Soil Air Water

当前位置： X-MOL 学术 › Clean - Soil Air Water › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Imaging and Identifying BSA Biofouling through the Combination of Fluorescence Microscopy and Flux Experiments
Clean - Soil Air Water ( IF 1.5 ) Pub Date : 2021-08-03 , DOI: 10.1002/clen.202000326
Charles S. Widing ₁ , Nathan R. Ruffle‐Deignan ₁ , Elana M.S. Stennett ₁

Affiliation

Biofouling decreases efficiency of membrane-based water filtration, like desalination, because fouling decreases flow rates. Understanding biofouling mechanism is crucial for increased implementation of desalination though few techniques for studying this exist. Flux experiments are combined with fluorescent membrane images to determine the biofouling mechanism of bovine serum albumin (BSA), a common model protein, with two mixed cellulose ester membranes. Samples containing 1% to 12% fluorescently tagged BSA (remaining protein unlabeled) are tested to determine the ideal amount of labeled protein. Due to large standard deviation in the fouling decays, the relative flux decays are fit for statistical analysis. No correlation between the decay and amount of labeled protein is found, suggesting the dye has little impact on the fouling. A 2.5% or 5% labeled protein sample is optimal to generate images and visualize the beginning of cake formation (blockage of membrane pores). By fitting the flux data to biofouling mechanism equations, it is concluded BSA deposits in the membrane pores eventually leading to cake formation. These results are corroborated using scanning electron microscopy images. Combining flux and fluorescence microscopy allows for further insight into biofouling mechanism and can be applied to other biological molecules to improve filtration techniques.

中文翻译：

通过结合荧光显微镜和通量实验对 BSA 生物污垢进行成像和识别

生物污垢会降低基于膜的水过滤的效率，例如海水淡化，因为污垢会降低流速。了解生物污垢机制对于增加海水淡化的实施至关重要，尽管目前很少有研究这种机制的技术。通量实验与荧光膜图像相结合，以确定牛血清白蛋白 (BSA)（一种常见的模型蛋白）与两种混合纤维素酯膜的生物污染机制。测试含有 1% 至 12% 荧光标记 BSA（剩余蛋白质未标记）的样品，以确定理想的标记蛋白质量。由于污垢衰减的标准偏差很大，因此相对通量衰减适合于统计分析。没有发现衰变与标记蛋白量之间的相关性，表明染料对污染几乎没有影响。A2。5% 或 5% 标记的蛋白质样品是生成图像和可视化蛋糕形成开始（膜孔堵塞）的最佳选择。通过将通量数据与生物污染机理方程拟合，得出结论，BSA 沉积在膜孔中，最终导致滤饼形成。使用扫描电子显微镜图像证实了这些结果。结合通量和荧光显微镜可以进一步了解生物污染机制，并可应用于其他生物分子以改进过滤技术。使用扫描电子显微镜图像证实了这些结果。结合通量和荧光显微镜可以进一步了解生物污染机制，并可应用于其他生物分子以改进过滤技术。使用扫描电子显微镜图像证实了这些结果。结合通量和荧光显微镜可以进一步了解生物污染机制，并可应用于其他生物分子以改进过滤技术。

更新日期：2021-10-09

点击分享查看原文

点击收藏

阅读更多本刊最新论文