Abstract

The evaluation of climate plasticity and content of free amino acids, sugars and fatty acids in Bulgarian soybean cultivars were used as an innovative approach. The field performance, expression and metabolomic profiles of leaves, green seeds and mature seeds of plants grown from low temperature pre-treated and not-treated seeds were assessed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and gas chromatography/mass spectrometry (GC/MS). The data from the morphological indicators, expression profiles and content of metabolites confirmed that the plants grown from low temperature pre-treated seeds had better performance. The expression profiles correlated with the content of amino acids and sugars. Inclusion of extruded full-fat soybean from cvs. Srebrina and Richy in the compound feed for pigs and soybean cake obtained after cold pressed soybeans of cv. Srebrina in compound feed for laying hens were investigated. The results confirmed that extruded full-fat soybean cv. Srebrina could be successfully included in the protein component of feed for growing pigs at a rate of 50% of soybean meal in protein equivalent. Extruded full-fat soybean cv. Richy in the amount of 30% of the protein component of the ration, in protein equivalent of the compound feed for the period of fattening of pigs could be included. The results confirmed that feed for laying hens, in which 50% of imported soybean meal is replaced by soybean cake produced from locally grown soybean, could increase the content of free amino and fatty acids of the final product and benefit the quality of eggs.

Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1954092 .

摘要

保加利亚大豆品种的气候可塑性和游离氨基酸、糖和脂肪酸含量的评估被用作一种创新方法。通过实时定量逆转录聚合酶链反应 (qRT-PCR) 和气相色谱法评估了从低温预处理和未处理的种子中生长的植物的叶子、绿色种子和成熟种子的田间表现、表达和代谢组学特征/质谱（GC/MS）。形态指标、表达谱和代谢物含量的数据证实，由低温预处理的种子生长的植物具有更好的性能。表达谱与氨基酸和糖的含量相关。包含来自cvs的挤压全脂大豆. Srebrina 和 Richy 在猪的配合饲料和cv .冷榨大豆后获得的豆饼。对蛋鸡配合饲料中的 Srebrina 进行了研究。结果证实挤压全脂大豆cv . Srebrina 可以成功地包含在生长猪饲料的蛋白质成分中，蛋白质当量相当于豆粕的 50%。膨化全脂大豆品种. 可加入占日粮蛋白质成分30%、蛋白质当量的猪育肥期配合饲料。结果证实，将50%的进口豆粕替换为本地种植的大豆生产的豆饼的蛋鸡饲料可以增加最终产品中游离氨基酸和脂肪酸的含量，有利于鸡蛋的质量。

本文的补充数据可在 https://doi.org/10.1080/13102818.2021.1954092 在线获得。