Osteoporosis, one of the most serious public health concerns caused by an imbalance between bone resorption and bone formation, has a major impact on the population. Therefore, finding the effective osteogenic compounds for the treatment of osteoporosis is a promising research approach. In our study, tamarind (Tamarindus indica L.) seed polysaccharide (TSP) extracted from tamarind seed was subjected to synthesize its sulfate derivatives. The 1H NMR, FT-IR, SEM, monosaccharide compositions and elemental analysis data revealed that tamarind seed polysaccharide sulfate (TSPS) was successfully prepared. As the result, TSPS showed potent effects on inducing osteoblast differentiation via increasing alkaline phosphatase (ALP) activity up to 20% after 10 days and bone mineralization approximately 58% after four weeks at concentration of 20 μg/mL, whereas no statistically increase for both ALP activity and bone mineralization was observed in TSP treatment. Furthermore, TSPS enhanced expression of several marker genes in bone formation. Overall, the obtained data provided novelty on osteogenic compounds originated from TSP of T. indica, as well as scientific fundamentals on drug development and bone tissue engineering for the treatment of osteoporosis and other bone-related diseases.

中文翻译：

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罗望子多糖硫酸化衍生物的体外成骨活性。


骨质疏松症是由骨吸收和骨形成之间不平衡引起的最严重的公共卫生问题之一，对人口产生重大影响。因此，寻找治疗骨质疏松症的有效成骨化合物是一种有前途的研究方法。在我们的研究中，从罗望子种子中提取的罗望子（Tamarindus indica L.）种子多糖（TSP）被合成了其硫酸盐衍生物。 1H NMR、FT-IR、SEM、单糖组成和元素分析数据表明，成功制备了罗望子籽多糖硫酸盐（TSPS）。结果，TSPS 在 20 μg/mL 的浓度下，通过将碱性磷酸酶 (ALP) 活性在 10 天后提高高达 20%，在 4 周后将骨矿化率提高约 58%，从而显示出对诱导成骨细胞分化的有效作用，而两者均没有统计学上的增加在 TSP 治疗中观察到 ALP 活性和骨矿化。此外，TSPS 增强了骨形成中几个标记基因的表达。总体而言，所获得的数据为源自印度籼稻TSP的成骨化合物提供了新颖性，并为治疗骨质疏松症和其他骨相关疾病的药物开发和骨组织工程提供了科学基础。