Development and characterization of 13 microsatellite markers of Chanodichthys mongolicus (Cypriniformes: Cyprinidae) by RAD-seq,Journal of Applied Ichthyology

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Development and characterization of 13 microsatellite markers of Chanodichthys mongolicus (Cypriniformes: Cyprinidae) by RAD-seq
Journal of Applied Ichthyology ( IF 0.7 ) Pub Date : 2021-08-03 , DOI: 10.1111/jai.14253
K. Liu ₁ , X. Y. Feng ₁ , H. J. Ma ₁ , N. Xie ₁

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This study developed and characterized 13 novel polymorphic microsatellite markers from Mongolian redfin Chanodichthys mongolicus by restriction-site associated DNA sequencing (RAD-seq). Variability was tested on 36 individuals randomly collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 (MGB_1990, MGB_3456, MGB_6007, MGB_6539, MGB_7273, MGB_8632) to 9 (MGB_8632). Observed heterozygosity ranged from 0.000 (MGB_7273) to 0.972 (MGB_5057), whereas the expected heterozygosity ranged from 0.028 (MGB_6007) to 0.795 (MGB_4875). Polymorphism Information Content ranged from 0.027 (MGB_6007) to 0.755 (MGB_4875). Moreover, seven microsatellites were in agreement with Hardy-Weinberg equilibrium. Four pairwise tests in seven microsatellite loci (MGB_1498, MGB_1990, MGB_4875, MGB_5057, MGB_6539, MGB_7273, MGB_8632) indicated linkage disequilibrium among loci. These microsatellites can evaluate Mongolian redfin populations’ genetic parameters to provide precious information for a conservation management system.

更新日期：2021-08-03

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