Low- and no-calorie sweeteners (LNCS) are food additives that have been widely consumed for many decades. Their safety has been well established by authoritative bodies globally and is re-evaluated periodically. The objective herein was to survey and summarize the genotoxicity potential of five commonly utilized LNCS: acesulfame potassium (Ace-K), aspartame, saccharin, steviol glycosides and sucralose. Data from peer-reviewed literature and the ToxCast/Tox21 database were evaluated and integrated with the most recent weight-of-evidence evaluations from authoritative sources. Emphasis was placed on assays most frequently considered for hazard identification and risk assessment: mutation, clastogenicity and/or aneugenicity, and indirect DNA damage, such as changes in DNA repair mechanisms or gene expression data. These five sweeteners have been collectively evaluated in hundreds of in vivo or in vitro studies that employ numerous testing models, many of which have been conducted according to specific testing guidelines. The weight-of-evidence demonstrates overall negative findings across assay types for each sweetener when considering the totality of study design, reliability and reporting quality, as well as the lack of carcinogenic responses (or lack of responses relevant to humans) in animal cancer bioassays as well as observational studies in humans. This conclusion is consistent with the opinions of authoritative sources that have consistently determined that these sweeteners lack mutagenic and genotoxic potential.

低热量和无热量甜味剂 (LNCS) 是几十年来被广泛食用的食品添加剂。它们的安全性已得到全球权威机构的充分肯定，并会定期重新评估。本文的目的是调查和总结五种常用 LNCS 的遗传毒性潜力：乙酰磺胺酸钾 (Ace-K)、阿斯巴甜、糖精、甜菊糖苷和三氯蔗糖。来自同行评审文献和 ToxCast/Tox21 数据库的数据经过评估，并与来自权威来源的最新证据权重评估相结合。重点放在最常用于危害识别和风险评估的分析上：突变、致裂性和/或无胚胎性，以及间接 DNA 损伤，例如 DNA 修复机制或基因表达数据的变化。采用多种测试模型的体内或体外研究，其中许多模型是根据特定的测试指南进行的。考虑到研究设计、可靠性和报告质量的整体，以及在动物癌症生物测定中缺乏致癌反应（或缺乏与人类相关的反应）时，证据权重表明每种甜味剂的检测类型的总体阴性结果以及对人类的观察性研究。这一结论与权威来源的观点一致，他们一致认为这些甜味剂没有诱变和遗传毒性的潜力。