The SD was extracted with a new green eutectic solvent, and the extraction method of TCM decoction was developed. In the quantitative analysis by HPLC, choline chloride phenol was selected as the eutectic solvent, THF was used as the extractant, and investigation of DES type, DES molar ratio, DES-to-THF ratio, vortex time, and material-to-liquid ratio was carried out. The experimental results showed that the optimal extraction method was as follows: the molar ratio of DES was 1 : 3, and the material-liquid ratio was 5 : 1200 (mL/μL). The volume ratio of DES to THF was 1200 : 800 (μL), the vortex time was 3 min, and the extraction was repeated two times. The eutectic solvent liquid phase microextraction method was adopted to optimize the extraction method of SD and reduce the complicated processing, long time, and low efficiency of traditional methods. At the same time, in the mouse ammonia water inducing cough and phenol red excretion and expectorant experiments, SD high- and medium-dose groups have a significant inhibitory effect on the frequency of antitussive in mice and both can increase the excretion of phenol red to varying degrees, indicating that SD has good cough-relieving and expectorant effect. The present study suggests a scientific basis and basis for the clinical research and quality standard formulation of SD.

中文翻译：

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深共熔溶剂液相微萃取对桑白皮汤质量标准及镇咳祛痰作用的研究

用新型绿色共晶溶剂提取SD，开发了中药汤剂的提取方法。HPLC定量分析选择氯化胆碱苯酚为共晶溶剂，THF为萃取剂，考察DES类型、DES摩尔比、DES-THF比、涡旋时间和料液比例进行了。实验结果表明，最佳提取方法为：DES的摩尔比为1：3，并且该材料液比为5：1200（毫升/ μ L）。DES 与 THF 的体积比为 1200 : 800 ( μL)，涡旋时间为3 min，重复提取2次。采用共晶溶剂液相微萃取法优化SD的萃取方法，减少传统方法处理复杂、时间长、效率低的问题。同时，在小鼠氨水诱导咳嗽和酚红排泄祛痰实验中，SD高、中剂量组对小鼠镇咳药频率有显着抑制作用，均能增加酚红排泄不同程度，说明SD具有良好的止咳祛痰作用。本研究为SD的临床研究和质量标准制定提供了科学依据和依据。