Excessive prenatal opioid exposure may lead to the development of Neonatal Opioid Withdrawal Syndrome (NOWS). RNA-seq was done on 64 formalin-fixed paraffin-embedded placental tissue samples from 32 mothers with opioid use disorder, with newborns with NOWS that required treatment, and 32 prenatally unexposed controls. We identified 93 differentially expressed genes in the placentas of infants with NOWS compared to unexposed controls. There were 4 up- and 89 downregulated genes. Among these, 7 genes CYP1A1, APOB, RPH3A, NRXN1, LINC01206, AL157396.1, UNC80 achieved an FDR p-value of <0.01. The remaining 87 genes were significant with FDR p-value <0.05. The 4 upregulated, CYP1A1, FP671120.3, RAD1, RN7SL856P, and the 10 most significantly downregulated genes were RNA5SP364, GRIN2A, UNC5D, DMBT1P1, MIR3976HG, LINC02199, LINC02822, PANTR1, AC012178.1, CTNNA2. Ingenuity Pathway Analysis identified the 7 most likely to play an important role in the etiology of NOWS. Our study expands insights into the genetic mechanisms of NOWS development.

产前过量的阿片类药物暴露可能导致新生儿阿片类药物戒断综合征 (NOWS) 的发展。RNA-seq 对来自 32 名患有阿片类药物使用障碍的母亲的 64 份福尔马林固定石蜡包埋的胎盘组织样本进行 RNA-seq，其中包括需要治疗的 NOWS 新生儿和 32 名产前未暴露的对照。与未暴露的对照组相比，我们在 NOWS 婴儿的胎盘中鉴定了 93 个差异表达的基因。有4个上调基因和89个下调基因。其中，7个基因CYP1A1 、APOB 、RPH3A、 NRXN1 、LINC01206、AL157396.1、UNC80达到<0.01的FDR p值。其余 87 个基因具有显着性，FDR p 值 <0.05。4个上调，CYP1A1、FP671120.3、RAD1、RN7SL856P， 10 个最显着下调的基因是RNA5SP364、GRIN2A 、UNC5D、DMBT1P1、MIR3976HG、LINC02199、LINC02822、PANTR1、AC012178.1、CTNNA2。Ingenuity Pathway Analysis 确定了 7 个最有可能在NOWS 的病因学中发挥重要作用的因素。我们的研究扩展了对NOWS发展的遗传机制的见解。