Hydrophobins are small, secreted proteins with important physiological functions in mycelial growth and fungal development. Here, 1 nucleus-specific and 35 allelic hydrophobin genes were identified in the genome of a white rot fungus, Coriolopsis trogii. Among these, 22 were eight-cysteine class I hydrophobin genes and the other 14 were uncommon six-cysteine hydrophobin genes. The six-cysteine hydrophobins were speculated to have originated from a common ancestor. The hydrophobin genes favored a clustering distribution and two recent duplication pairs were identified. The genes had conserved gene structures with three exons and two introns. Cthyd18, Cthyd19, and Cthyd32 were constitutively highly expressed in all developmental stages. Cthyd20, Cthyd21, Cthyd22, Cthyd28, Cthyd30, Cthyd31, and Cthyd33 were highly expressed in mycelia, and Cthyd12 and Cthyd35 in the reproductive stages. Sixteen hydrophobin genes were regulated differently in the transition from mycelia to primordia; Cthyd35 showed maximal upregulation of 1922-fold, and Cthyd23 showed maximal downregulation of 552-fold. Most (32) hydrophobin genes showed significant differential expression between mycelia cultured in different media (potato dextrose agar or broth). Weighted gene co-expression network analysis and promoter analysis revealed that C2H2 zinc finger proteins may regulate hydrophobin genes. These results may support further research into the function and evolution of hydrophobins.

疏水蛋白是小的分泌蛋白，在菌丝体生长和真菌发育中具有重要的生理功能。在这里，在白腐真菌Coriolopsis trogii的基因组中鉴定了 1 个核特异性和 35 个等位基因疏水蛋白基因。其中，22个为8-半胱氨酸I类疏水蛋白基因，另外14个为罕见的6-半胱氨酸疏水蛋白基因。据推测，六种半胱氨酸疏水蛋白起源于一个共同的祖先。疏水蛋白基因有利于聚类分布，并确定了两个最近的重复对。这些基因具有保守的基因结构，具有三个外显子和两个内含子。Cthyd18、Cthyd19和Cthyd32在所有发育阶段都组成性地高度表达。Cthyd20、Cthyd21、Cthyd22、Cthyd28、Cthyd30、Cthyd31和Cthyd33在菌丝体中高表达，Cthyd12和Cthyd35在繁殖期高表达。16个疏水蛋白基因在菌丝体向原基转变过程中受到不同的调控；Cthyd35显示出 1922 倍的最大上调，而Cthyd23显示最大下调 552 倍。大多数 (32) 疏水蛋白基因在不同培养基（马铃薯葡萄糖琼脂或肉汤）中培养的菌丝体之间显示出显着差异表达。加权基因共表达网络分析和启动子分析表明，C2H2 锌指蛋白可能调节疏水蛋白基因。这些结果可能支持进一步研究疏水蛋白的功能和进化。