Abstract—

The work was aimed at detection of potential degraders of polyethylene terephthalate (PET), polystyrene (PS), and steel 20 based on comparison of the taxonomic composition of the biofilm communities formed on these materials immersed in aqueous environments of diverse origin (marine, natural freshwater, and industrial) in different climatic regions. The libraries obtained by high-throughput sequencing of the 16S rRNA gene V3–V4 region were mainly grouped according to the media types. The composition and relative abundance of the taxa (both at the phylum/class and the genus levels) differed significantly between the groups of libraries; within the groups, these parameters varied depending mainly on the incubation time and on antimicrobial treatment (for industrial samples), while the material of the samples had less effect. Our results indicated that industrial recirculated water was more aggressive against polymer materials, with potential degraders of the polymers (PET and PS) belonging to the genera Pseudomonas, Acidovorax, and Arenimonas. Iron- and sulfur-oxidizing bacteria of the genera Sulfurimonas and Mariprofundus are probably involved in steel biocorrosion in seawater.

中文翻译：

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暴露于水环境的各种材料上细菌污染的分类组成的比较分析

摘要——

这项工作旨在检测聚对苯二甲酸乙二醇酯 (PET)、聚苯乙烯 (PS) 和钢 20 的潜在降解剂，基于对这些材料浸入不同来源（海洋、天然淡水和工业）在不同的气候区域。对 16S rRNA 基因 V3-V4 区进行高通量测序获得的文库主要根据培养基类型进行分组。库组之间的分类群的组成和相对丰度（在门/类和属水平上）存在显着差异；在组内，这些参数主要取决于孵育时间和抗菌处理（对于工业样品），而样品的材料影响较小。假单胞菌属、食酸菌属和沙单胞菌属。Sulfurimonas和Mariprofundus属的铁和硫氧化细菌可能与海水中的钢铁生物腐蚀有关。