Lycium barbarum have received an increasing popularity due to its powerful biological activity and medicinal use. However, the effect of Lycium barbarum on skin remains largely uncharacterized. The general purpose of this paper was to characterize the phenolic compounds in Lycium barbarum extract (LBE) using LC-HRMS/QTOF method and to investigate whether topical administration of LBE can repair skin barrier dysfunction in mice. Our data demonstrated that LBE could not only decrease ROS level and matrix metalloproteinase expression, but also strengthen intrinsic antioxidant defense system including SOD, GSH-Px and CAT, thereby resulting in increased skin collagen content and an improvement of UV-induced skin erythema, thickness and wrinkles. Improved skin barrier functions were highly correlated with increased expression of filaggrin, involucrin and loricrin as well as antioxidant proteins such as Nrf2 and HO-1 in UV-irradiated mice, suggesting that LBE may be promising natural products at a lower cost for the topical application in the treatment of skin diseases with defective barrier function.

中文翻译：

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枸杞酚类提取物通过Nrf2/HO-1调控改善皮肤屏障功能障碍

枸杞因其强大的生物活性和药用价值而越来越受欢迎。然而，枸杞对皮肤的影响在很大程度上仍然没有定论。本文的总体目的是表征枸杞中的酚类化合物。使用 LC-HRMS/QTOF 方法提取 (LBE) 并研究 LBE 局部给药是否可以修复小鼠皮肤屏障功能障碍。我们的数据表明，LBE 不仅可以降低 ROS 水平和基质金属蛋白酶的表达，还可以增强包括 SOD、GSH-Px 和 CAT 在内的内在抗氧化防御系统，从而增加皮肤胶原蛋白含量，改善紫外线引起的皮肤红斑、厚度和皱纹。改善的皮肤屏障功能与紫外线照射小鼠中丝聚蛋白、外皮蛋白和 loricrin 以及 Nrf2 和 HO-1 等抗氧化蛋白的表达增加高度相关，这表明 LBE 可能是一种有前景的天然产品，成本较低，可用于局部应用用于治疗屏障功能缺陷的皮肤病。