Terminalia avicennioides Guill and Perr (Combretaceae) is an important West African medicinal plant. The plant is used locally against microbes and parasites in both humans and animals and studies have demonstrated its cytotoxicity potential. Thus, this study was carried out to test the cytotoxic effect of the extracts and fractions of the root of the medicinal plant Terminalia avicennioides Guill and Perr (Combretaceae) in two different cell lines. Methanol, ethanol, 30 % ethanol, hot water and cold water extracts and ethylacetate, hexane, chloroform, butanol and residual water fractions, were evaluated at 1000, 750, 500, 250, 100 and 50 µg/mL concentrations, with doxorubicin as positive control. The cells were incubated with the extracts for 48 h at 37 °C in a 5 % CO2 humidified incubator. The inhibition of cell viability, determined with the methyl blue thiazole tetrazolium bromide (MTT) assay, was used to assess the anti-proliferative effect of the extracts, in normal Vero Monkey kidney and human liver cancer (HepG2) cell lines. There was a concentration-dependent inhibition of cell viability in both the HepG2 and Vero cell lines. For HepG2 cells, antiproliferative effect was highest for the hexane fraction (viability ranged from 19.63 ± 1.10 % to 70.30 ± 1.78 % for 1000 and 50 µg/mL, respectively. For Vero cells, the highest antiproliferative effect, at 1000 µg/mL, was with hexane fraction (cell viability 21.37 ± 3.50 %), while at 50 µg/mL the chloroform fraction demonstrated the highest effect (viability of 86.10 ± 1.95 %). The extracts and fractions from the root of Terminalia avicennioides have antiproliferative effect on the Vero and HepG2 cell lines tested. However, the extracts and fractions were not more toxic to the HepG2 than to the Vero cells. The cytotoxic effect of stem-bark and leaf extracts could be evaluated in the future to determine its anticancer potential.

中文翻译：

---

榄仁树（Combretaceae）Guill 和Perr 根提取物和组分的抗增殖作用。在 HepG2 和 Vero 细胞系上

Terminalia avicennioides Guill and Perr (Combretaceae) 是一种重要的西非药用植物。该植物用于局部对抗人类和动物的微生物和寄生虫，研究表明其具有细胞毒性潜力。因此，进行这项研究是为了测试药用植物榄仁树和 Perr（Combretaceae）的根提取物和级分在两种不同细胞系中的细胞毒性作用。甲醇、乙醇、30% 乙醇、热水和冷水提取物以及乙酸乙酯、己烷、氯仿、丁醇和残留水馏分在 1000、750、500、250、100 和 50 µg/mL 浓度下进行评估，阿霉素为阳性控制。将细胞与提取物在 37°C 下在 5% CO2 加湿培养箱中培养 48 小时。抑制细胞活力，用甲基蓝噻唑溴化四唑 (MTT) 测定法测定的 α 用于评估提取物在正常 Vero 猴肾和人肝癌 (HepG2) 细胞系中的抗增殖作用。在 HepG2 和 Vero 细胞系中都存在浓度依赖性的细胞活力抑制。对于 HepG2 细胞，正己烷组分的抗增殖作用最高（活力范围分别为 19.63 ± 1.10 % 至 70.30 ± 1.78 %，浓度为 1000 和 50 µg/mL。对于 Vero 细胞，抗增殖作用最高，浓度为 1000 µg/mL，使用己烷组分（细胞活力 21.37 ± 3.50 %），而在 50 µg/mL 氯仿组分表现出最高的效果（活力为 86.10 ± 1.95 %）。测试了 Vero 和 HepG2 细胞系。然而，提取物和级分对 HepG2 的毒性并不比对 Vero 细胞的毒性大。未来可以评估茎皮和叶提取物的细胞毒性作用，以确定其抗癌潜力。