Hybrid nanoparticles composed of different biopolymers for delivery of enzyme/prodrug systems are of interest for cancer therapy. Hyaluronic acid-coated chitosan nanoparticles (CS/HA NP) were prepared to encapsulate individually an enzyme/pro-drug complex based on horseradish peroxidase (HRP) and indole-3-acetic acid (IAA). CS/HA NP showed size around 158 nm and increase to 170 and 200 nm after IAA and HRP encapsulation, respectively. Nanoparticles showed positive zeta potential values (between +20.36 mV and +24.40 mV) and higher encapsulation efficiencies for both nanoparticles (up to 90 %) were obtained. Electron microscopy indicated the formation of spherical particles with smooth surface characteristic. Physicochemical and thermal characterizations suggest the encapsulation of HRP and IAA. Kinetic parameters for encapsulated HRP were similar to those of the free enzyme. IAA-CS/HA NP showed a bimodal release profile of IAA with a high initial release (72 %) followed by a slow-release pattern. The combination of HRP-CS/HA NP and IAA- CS/HA NP reduced by 88 % the cell viability of human bladder carcinoma cell line (T24) in the concentrations 0.5 mM of pro-drug and 1.2 μg/mL of the enzyme after 24 h.

由不同生物聚合物组成的用于递送酶/前药系统的混合纳米粒子对癌症治疗很感兴趣。制备了透明质酸包被的壳聚糖纳米颗粒 (CS/HA NP) 以单独封装基于辣根过氧化物酶 (HRP) 和吲哚-3-乙酸 (IAA) 的酶/前药复合物。CS/HA NP 显示尺寸约为 158 nm，并在 IAA 和 HRP 封装后分别增加到 170 和 200 nm。纳米颗粒显示出正 zeta 电位值（在 +20.36 mV 和 +24.40 mV 之间），并且获得了两种纳米颗粒的更高封装效率（高达 90%）。电子显微镜表明形成具有光滑表面特征的球形颗粒。物理化学和热特性表明 HRP 和 IAA 的封装。封装的 HRP 的动力学参数与游离酶的动力学参数相似。IAA-CS/HA NP 显示 IAA 的双峰释放曲线，初始释放高 (72%)，然后是缓释模式。HRP-CS/HA NP 和 IAA-CS/HA NP 的组合在浓度为 0.5 mM 的前药和 1.2 μg/mL 的酶后使人膀胱癌细胞系 (T24) 的细胞活力降低了 88% 24 小时。