Rapid quantitative assays for glucose-6-phosphate dehydrogenase (G6PD) and hemoglobin combined on a capillary-driven microfluidic chip,Lab on a Chip

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Rapid quantitative assays for glucose-6-phosphate dehydrogenase (G6PD) and hemoglobin combined on a capillary-driven microfluidic chip
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-07-27 , DOI: 10.1039/d1lc00354b
Marco Rocca _{1,

2} , Yuksel Temiz ₁ , Marie L Salva _{1,

2} , Samuel Castonguay ₃ , Thomas Gervais _{3,

4,

5} , Christof M Niemeyer ₂ , Emmanuel Delamarche ₁

Affiliation

Rapid tests for glucose-6-phosphate dehydrogenase (G6PD) are extremely important for determining G6PD deficiency, a widespread metabolic disorder which triggers hemolytic anemia in response to primaquine and tafenoquine medication, the most effective drugs for the radical cure of malaria caused by Plasmodium parasites. Current point-of-care diagnostic devices for G6PD are either qualitative, do not normalize G6PD activity to the hemoglobin concentration, or are very expensive. In this work we developed a capillary-driven microfluidic chip to perform a quantitative G6PD test and a hemoglobin measurement within 2 minutes and using less than 2 μL of sample. We used a powerful microfluidic module to integrate and resuspend locally the reagents needed for the G6PD assay and controls. We also developed a theoretical model that successfully predicts the enzymatic reactions on-chip, guides on-chip reagent spotting and allows efficient integration of multiple assays in miniaturized formats with only a few nanograms of reagents.

中文翻译：

在毛细管驱动的微流控芯片上对葡萄糖 6-磷酸脱氢酶 (G6PD) 和血红蛋白进行快速定量分析

葡萄糖-6-磷酸脱氢酶（G6PD）快速测试用于确定G6PD缺乏症，一种普遍的代谢疾病，其触发溶血性贫血响应于伯氨喹和他非诺喹药物，最有效的药物引起的疟疾的根治极其重要的疟原虫寄生虫。当前用于 G6PD 的即时诊断设备要么是定性的，要么无法将 G6PD 活性标准化为血红蛋白浓度，要么非常昂贵。在这项工作中，我们开发了一种毛细管驱动的微流控芯片，可在 2 分钟内执行定量 G6PD 测试和血红蛋白测量，并且使用少于 2 μL 的样品。我们使用功能强大的微流体模块在本地整合和重新悬浮 G6PD 检测和控制所需的试剂。我们还开发了一个理论模型，该模型成功地预测了芯片上的酶促反应，指导芯片上的试剂点样，并允许以仅几纳克试剂的小型化形式有效集成多种检测。

更新日期：2021-08-03

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