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Antibiotic susceptibility test under a linear concentration gradient using travelling surface acoustic waves
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-07-23 , DOI: 10.1039/d1lc00418b Yongtaek Im 1 , Seunggyu Kim 1 , Jinsoo Park 1, 2 , Hyung Jin Sung 1 , Jessie S Jeon 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-07-23 , DOI: 10.1039/d1lc00418b Yongtaek Im 1 , Seunggyu Kim 1 , Jinsoo Park 1, 2 , Hyung Jin Sung 1 , Jessie S Jeon 1
Affiliation
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An efficient and accurate antibiotic susceptibility test (AST) is indispensable for measuring the antimicrobial resistance of pathogenic bacteria. A minimal inhibitory concentration (MIC) can be obtained without performing repeated dilutions of the antibiotic by forming a linear antibiotic concentration gradient in a microfluidic channel. We demonstrated a device designed to use travelling surface acoustic waves (TSAWs) to enable a rapid formation of an antibiotic gradient in a few seconds. The TSAWs produced by a focused interdigital transducer deposited on the surface of a piezoelectric (LiNbO3) substrate generated an acoustic streaming flow inside a microfluidic channel, which mixed confluent streams of antibiotics in a controlled fashion. The growth of bacteria exposed to the antibiotic gradient was determined by measuring the MIC, which was used as an indicator of the effectiveness of the AST. The concentration gradient produced using our device was linear, a feature that enhanced the reliability of measurements throughout the microchannel. Two ASTs, namely Pseudomonas aeruginosa against gentamicin and levofloxacin were chosen for the case of slowly proliferating bacteria, and one AST, namely Escherichia coli against gentamicin, were chosen for the rapidly proliferating case. Appropriate antibiotic doses for Pseudomonas aeruginosa and Escherichia coli were each obtained in an efficient manner.
中文翻译：
使用行表面声波在线性浓度梯度下进行抗生素敏感性测试
有效且准确的抗生素药敏试验（AST）对于测量病原菌的抗菌素耐药性是必不可少的。通过在微流体通道中形成线性抗生素浓度梯度，无需重复稀释抗生素即可获得最小抑菌浓度 (MIC)。我们展示了一种设计用于使用行进表面声波 (TSAW) 的设备，可以在几秒钟内快速形成抗生素梯度。由沉积在压电材料 (LiNbO 3) 底物在微流体通道内产生声流，以受控方式混合融合的抗生素流。暴露于抗生素梯度的细菌的生长通过测量 MIC 来确定，MIC 用作 AST 有效性的指标。使用我们的设备产生的浓度梯度是线性的，这一特性提高了整个微通道测量的可靠性。两种AST，即铜绿假单胞菌对抗庆大霉素和左氧氟沙星被选择用于缓慢增殖的细菌的情况，而一种AST，即大肠杆菌对抗庆大霉素的情况被选择用于快速增殖的情况。铜绿假单胞菌的适当抗生素剂量和大肠杆菌均以有效方式获得。
更新日期：2021-08-03
中文翻译：
使用行表面声波在线性浓度梯度下进行抗生素敏感性测试
有效且准确的抗生素药敏试验（AST）对于测量病原菌的抗菌素耐药性是必不可少的。通过在微流体通道中形成线性抗生素浓度梯度，无需重复稀释抗生素即可获得最小抑菌浓度 (MIC)。我们展示了一种设计用于使用行进表面声波 (TSAW) 的设备，可以在几秒钟内快速形成抗生素梯度。由沉积在压电材料 (LiNbO 3) 底物在微流体通道内产生声流，以受控方式混合融合的抗生素流。暴露于抗生素梯度的细菌的生长通过测量 MIC 来确定，MIC 用作 AST 有效性的指标。使用我们的设备产生的浓度梯度是线性的，这一特性提高了整个微通道测量的可靠性。两种AST，即铜绿假单胞菌对抗庆大霉素和左氧氟沙星被选择用于缓慢增殖的细菌的情况，而一种AST，即大肠杆菌对抗庆大霉素的情况被选择用于快速增殖的情况。铜绿假单胞菌的适当抗生素剂量和大肠杆菌均以有效方式获得。
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