Understanding the soil dissolved organic matter (DOM) chemodiversity and its interaction with microbes is crucial to comprehend the biogeochemical processes in soil. In this study, ultrahigh-resolution fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was employed to in detail characterize the DOM chemodiversity at a molecular level. The variation of the DOM chemodiversity from four agro-ecological experimental sites (paddy fields) subjected to different long-term fertilizations across different distance-points was evaluated. Geographic distance had a greater impact on DOM chemodiversity than anthropogenic fertilization. Distance-decay analysis showed that the dissimilarity of the DOM chemodiversity significantly increased with the increase in geographic distance. Long-term organic fertilizations homogenized the DOM chemodiversity as it made lipid-like compounds more similar regardless of geography. A network analysis combining DOM chemodiversity and bacterial community composition showed the significant interactions between bacteria species and DOM molecules. Variable partitioning analysis (VPA) showed that the bacterial community diversity dictates the soil DOM chemodiversity and not vice versa. Geographic distance indirectly affects the soil DOM chemodiversity by shaping the bacterial community.

了解土壤溶解有机质 (DOM) 化学多样性及其与微生物的相互作用对于理解土壤中的生物地球化学过程至关重要。在这项研究中，采用超高分辨率傅立叶变换离子回旋共振质谱 (FT-ICR-MS) 在分子水平上详细表征 DOM 化学多样性。评估了在不同距离点进行不同长期施肥的四个农业生态试验点（稻田）的 DOM 化学多样性的变化。与人为施肥相比，地理距离对 DOM 化学多样性的影响更大。距离衰减分析表明，随着地理距离的增加，DOM化学多样性的差异性显着增加。长期的有机施肥使 DOM 化学多样性均质化，因为它使脂质类化合物更加相似，无论地理位置如何。结合 DOM 化学多样性和细菌群落组成的网络分析显示细菌物种和 DOM 分子之间存在显着的相互作用。变量分区分析 (VPA) 表明，细菌群落多样性决定了土壤 DOM 化学多样性，反之亦然。地理距离通过塑造细菌群落间接影响土壤 DOM 化学多样性。变量分区分析 (VPA) 表明，细菌群落多样性决定了土壤 DOM 化学多样性，反之亦然。地理距离通过塑造细菌群落间接影响土壤 DOM 化学多样性。变量分区分析 (VPA) 表明，细菌群落多样性决定了土壤 DOM 化学多样性，反之亦然。地理距离通过塑造细菌群落间接影响土壤 DOM 化学多样性。