Abstract

Purpose

In the present clinical study, it was aimed to investigate the possible effects of Trypan blue (TB) use on the corneal endothelium during cataract surgery in eyes with pseudoexfoliation syndrome (PEX) during a three-month follow-up period using the contralateral eye control design.

Methods

This prospective, randomised controlled, individual cohort study included 92 eyes of 46 patients with bilateral PEX and cataracts. While 1% TB was applied to one eye of the patients before capsulorhexis (study group), it was not applied to the other eye (control group). Both groups were compared preoperatively and postoperatively in terms of endothelial cell density (ECD), endothelial cell loss (%), pleomorphism, polymegathism and central corneal thickness (CCT) using specular microscopy.

Results

Preoperative corneal ECD was measured as 2362.56 ± 253.27 in the study group, 2380.84 ± 220.54 in the control group, and 2145.58 ± 221.71 in the study group and 2184.97 ± 200.94 cells/mm² in the control group in the postoperative 3rd-month follow-up (p = 0.71 and = 0.37, respectively). In addition, there were no significant differences between the two groups in terms of the percentage of hexagonal cells, coefficient of variation (CV), and CCT both preoperatively and postoperatively 3 months later (p = 0.78, =0.39, =0.95 preoperatively and p = 0.31, =0.26, =0.83 postoperatively, respectively).

Conclusion

This study demonstrated that the injection of 1% TB into the anterior chamber for staining the anterior capsule during cataract surgery did not cause significant corneal endothelial changes at postoperative 3^rd months, despite the increased fragility of corneal endothelial cells in patients with PEX.

中文翻译：

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台盼蓝对假性剥脱综合征 (PEX) 患者白内障手术期间角膜内皮的影响

摘要

目的

在本临床研究中，旨在使用对侧眼部对照进行为期三个月的随访，研究在假性剥脱综合征 (PEX) 眼白内障手术期间使用台盼蓝 (TB) 对角膜内皮的可能影响设计。

方法

这项前瞻性、随机对照、个体队列研究包括 46 名双侧 PEX 和白内障患者的 92 只眼睛。虽然在撕囊前将 1% TB 应用于患者的一只眼睛（研究组），但未将其应用于另一只眼睛（对照组）。使用镜面显微镜比较两组术前和术后的内皮细胞密度（ECD）、内皮细胞丢失（%）、多形性、多聚性和中央角膜厚度（CCT）。

结果

术后第3个月随访，研究组术前角膜ECD为2362.56±253.27，对照组为2380.84±220.54，研究组为2145.58±221.71，对照组为2184.97±200.94个细胞/mm ² 。向上（分别为p  = 0.71 和 = 0.37）。此外，两组在术前和术后 3 个月的六角形细胞百分比、变异系数（CV）和 CCT 方面均无显着差异（p  = 0.78, =0.39, =0.95 术前和p  = 0.31，= 0.26，= 0.83 术后，分别）。

结论

这项研究表明，尽管 PEX 患者角膜内皮细胞的脆性增加，但在白内障手术期间向前房注射 1% TB 用于对前囊进行染色不会在术后第 3 个月引起明显的角膜内皮变化^。