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Phenotypic and marker-assisted characterization of new apple genotypes with high resistance to fire blight
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2021-05-31 , DOI: 10.1007/s10658-021-02303-x
Piotr Sobiczewski , Sylwia Keller-Przybyłkowicz , Mariusz Lewandowski , Artur Mikiciński , Robert Maciorowski

In the period 2016–2018 the susceptibility to fire blight of seven apple genotypes, including four recently bred was assessed. The studies showed that two of the genotypes (J-2003-05 and ‘Early Szampion’) are highly resistant to the disease. The indicator of assessment was evaluated by the size of necrotized part of terminal shoots after their inoculation with strain Ea659 of Erwinia amylovora. The analysis of disease incidence on individual shoots of both genotypes showed no symptoms on 92% and 100% of shoots inoculated in 2016, 83.8 and 77.0% of shoots in 2017, and 87.8 and 77.3% of shoots inoculated in 2018, respectively. Molecular tests based on 16 genetic markers revealed that genotype J-2003-05 possesses 10 resistance alleles (covering the region of interest from LG 3, 5, 7, 10 and 12). This genotype is derived from the cross between ‘Melfree’ and ‘Sawa’ and produces very large dark-red tasty fruits. Genotype ‘Early Szampion’ representing the selected hybrid from the cross of ‘Gold Milenium’ and ‘Šampion’, producing large, bright red, attractive fruits, was found to possess 10 resistance alleles (covering the region of interest from LG 3, 7, 10 and 12), Both genotypes have the prospect of being used in breeding programs and commercial planting.



中文翻译:

高抗火疫病苹果新基因型的表型和标记辅助表征

在 2016-2018 年期间,评估了七种苹果基因型对火疫病的易感性,其中包括四种最近培育的。研究表明,其中两种基因型(J-2003-05 和“Early Szampion”)对该病具有高度抵抗力。评价指标通过接种梨火疫病菌Ea659菌株后的末端枝条坏死部分的大小来评价。. 对两种基因型的单个枝条发病率的分析显示,2016 年接种的枝条分别为 92% 和 100%,2017 年接种的枝条为 83.8% 和 77.0%,2018 年接种的枝条为 87.8% 和 77.3%。基于 16 个遗传标记的分子测试显示,基因型 J-2003-05 具有 10 个抗性等位基因(涵盖 LG 3、5、7、10 和 12 的感兴趣区域)。这种基因型源自“Melfree”和“Sawa”之间的杂交,产生非常大的深红色美味水果。基因型 'Early Szampion' 代表从 'Gold Milenium' 和 'Šampion' 杂交选出的杂交种,生产大而鲜红色的诱人果实,被发现具有 10 个抗性等位基因(覆盖 LG 3、7、 10 和 12),

更新日期:2021-08-03
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