The stem nematode, Ditylenchus dipsaci, is a severe pest in European sugar beet production. In France, Germany, and Switzerland, D. dipsaci damage in sugar beet varies among specific geographic areas. In this study, the reproduction potential of four geographically distinct D. dipsaci populations was determined using sterile carrot disc cultures. In addition, virulence and pathogenicity were investigated in-vivo using sugar beet. No difference was found in the reproduction potential on carrot discs, as well as penetration rate in sugar beet seedlings. The reproduction rate in sugar beet tissue was significantly affected by the D. dipsaci population used. The population from Seeland (CH) showed the highest number of nematodes per plant at 60 dpi (21,071.8 ± 5340.0), compared to the three other populations contained 3588.6 ± 3858.3, 5136.9 ± 4950.8, and 3579.7 ± 5174.2, respectively. Furthermore, the reproduction rate of D. dipsaci was negatively correlated with fresh biomass of sugar beets at 60 dpi. Based on these results, the D. dipsaci population “Seeland” is suitable for breeding programs to detect resistance in sugar beet. After selecting candidate genotypes/varieties, these should be further evaluated for their field resistance in their targeted growing regions.

茎线虫Ditylenchus dipsaci是欧洲甜菜生产中的一种严重害虫。在法国、德国和瑞士，甜菜中鳞球茎线虫的损害因特定地理区域而异。在这项研究中，使用无菌胡萝卜圆盘培养物确定了四个地理上不同的鳞球茎线虫种群的繁殖潜力。此外，使用甜菜在体内研究了毒力和致病性。在胡萝卜圆盘上的繁殖潜力以及在甜菜幼苗中的渗透率方面没有发现差异。甜菜组织中的繁殖率受鳞球茎线虫的显着影响使用的人口。来自 Seeland (CH) 的种群在 60 dpi (21,071.8 ± 5340.0) 时每株植物的线虫数量最高，而其他三个种群分别包含 3588.6 ± 3858.3、5136.9 ± 4950.8 和 3579.7 ± 51. 此外，鳞球茎的繁殖率与 60 dpi 的甜菜新鲜生物量呈负相关。基于这些结果，鳞球茎线虫种群“Seeland”适合用于检测甜菜抗性的育种计划。选择候选基因型/品种后，应进一步评估它们在目标生长区域的田间抗性。