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Formulated Ampelomyces quisqualis CPA-9 applied on zucchini leaves: influence of abiotic factors and powdery mildew mycoparasitization
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2021-05-27 , DOI: 10.1007/s10658-021-02302-y
Anna Carbó , Neus Teixidó , Josep Usall , Cristina Solsona , Rosario Torres

Even though the potential of the fungus Ampelomyces quisqualis against powdery mildew has been largely demonstrated, the efficacy of the commercialised product AQ10 Biofungicide is not consistent. Recently, a solid formulation of A. quisqualis strain CPA-9 that included biodegradable coatings on its composition was developed to overcome the major shortcomings of the biocontrol agents applied under practical conditions. The aims of the present study were to show the compatibility of CPA-9 with different phytosanitary products, and to confirm the potential of this novel formulation in different approaches: (i) under different conditions of temperature (20 and 30 °C) and relative humidity (40, 60 and 85%) on different surfaces (glass and zucchini leaves), (ii) after different rainfall episodes, and (iii) verifying Podosphaera xanthii parasitization by dried conidia of CPA-9. It was demonstrated that CPA-9 was compatible with several phytosanitary products, so it might be included in integrated management programmes. Moreover, the solid formulation showed better resilience than non-formulated conidia, both applied on a glass surface and on zucchini leaves. Adherence of both treatments on zucchini leaves did not show significant differences after simulated rainfall and all tested concentrations of dried conidia were able to produce pycnidia in P. xanthii hyphae. Therefore, the developed fluidised-bed spray-dried formulation of A. quisqualis CPA-9 together with coatings compounds has all the makings of becoming a biocontrol product, although their efficacy under practical conditions should be assessed.



中文翻译:

西葫芦叶上应用的配方 Ampelomyces quisqualis CPA-9:非生物因素和白粉病真菌寄生的影响

尽管真菌Ampelomyces quisqualis对抗白粉病的潜力已在很大程度上得到证明,但商业化产品 AQ10 生物杀菌剂的功效并不一致。最近,A. quisqualis的固体制剂菌株 CPA-9 在其成分上包含可生物降解的涂层,以克服在实际条件下应用的生物防治剂的主要缺点。本研究的目的是展示 CPA-9 与不同植物检疫产品的相容性,并确认这种新配方在不同方法中的潜力:(i) 在不同的温度条件(20 和 30°C)和相对不同表面(玻璃和西葫芦叶)的湿度(40%、60% 和 85%),(ii) 不同降雨事件后,以及 (iii) 验证Podosphaera xanthiiCPA-9 的干燥分生孢子寄生。经证明,CPA-9 与多种植物检疫产品兼容,因此可以将其纳入综合管理计划。此外,固体制剂表现出比非制剂分生孢子更好的弹性,无论是施用于玻璃表面还是西葫芦叶上。在模拟降雨后,两种处理对西葫芦叶的粘附性没有显示出显着差异,并且所有测试浓度的干燥分生孢子都能在P. xanthii菌丝中产生分生孢子。因此,开发的A. quisqualis CPA-9流化床喷雾干燥制剂与涂料化合物一起具有成为生物防治产品的所有条件,尽管应评估它们在实际条件下的功效。

更新日期:2021-08-03
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