Worldwide, most isolates of Macrophomina (Botryosphaeriaceae) have been attributed to the generalist phytopathogen M. phaseolina. Since 2014, three cryptic species of Macrophomina have been recognised by molecular methods. This study elucidates the taxonomy of Macrophomina species associated with broadacre and horticultural crops in Australia. A five-locus phylogenetic analysis of 80 isolates of Macrophomina from 28 plant species in Australia, combined with genealogical concordance phylogenetic species recognition and coalescent-based species delimitation approaches, found M. phaseolina, M. pseudophaseolina and supported the introduction of M. tecta sp. nov. Macrophomina phaseolina was the most frequently isolated (88%). Macrophomina pseudophaseolina is reported for the first time in Australia. Macrophomina tecta was isolated from stems of Sorghum bicolor and Vigna radiata with charcoal rot symptoms in New South Wales and Queensland. The potential for two or more Macrophomina species to co-infect the same host has implications for disease epidemiology and pathogen evolution. Future investigations into the distribution, biology, host range and population diversity of the new Macrophomina records are needed.

在世界范围内，大多数巨噬菌属（Botryosphaeriaceae）分离株被归于多面性植物病原体M. phaseolina。自 2014 年以来，已通过分子方法识别了巨噬菌属的三个隐匿物种。这项研究阐明的分类Macrophomina在澳大利亚广亩和园艺作物相关物种。对澳大利亚 28 种植物中的 80 株大角螟进行的五位点系统发育分析，结合谱系一致性系统发育物种识别和基于聚结的物种划分方法，发现了M. phaseolina、M.pseudophaseolina并支持引入M. tecta sp. 十一月 豆豉是最常分离的 (88%)。Macrophhomina pseudophaseolina在澳大利亚首次被报道。Macrophomina tecta从新南威尔士和昆士兰具有木炭腐烂症状的双色高粱和豇豆的茎中分离出来。两种或多种巨噬细胞共感染同一宿主的可能性对疾病流行病学和病原体进化具有影响。未来需要对新的巨目动物记录的分布、生物学、寄主范围和种群多样性进行调查。