Gelatin and N-methacryloyl-(L)-histidine methyl ester [MAH] containing poly(2-hydroxyethyl methacrylate) [PHEMA] based cryogels were prepared to aid in enhancing cell proliferation. In order to evaluate effects of MAH comonomer and gelatin, cryogels were prepared containing different amounts of gelatin and MAH. Accordingly, four different groups of cryogels which were PHEMA, poly(2-hydroxyethyl methacrylate)-gelatin [PHEMA/GEL], poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [PHEMAH] and poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester)-gelatin [PHEMAH/GEL] were prepared and a total of eight cryogel groups were obtained depending on the varying gelatin ratios. Swelling tests, scanning electron microscope (SEM), microcomputed tomography (Micro-CT) and Fourier transform infrared spectroscopy- Attenuated Total Reflectance (FTIR-ATR) were performed for characterization studies. FTIR-ATR studies confirmed that gelatin and MAH were incorporated into the cryogel structures. Average pore size of cryogels was in the range of 18−198 μm confirmed by Micro-CT and SEM analysis. Then, cryogels were evaluated for cell proliferation abilities by MTT analysis for 24 h and 48 h for cell viability in L929 mouse fibroblast cells. Cell viability of PHEMAH/0.2GEL cryogels reached 209 %. Interaction and proliferation of fibroblast cells on cryogels demonstrated by SEM examination.

制备明胶和N-甲基丙烯酰-(L)-组氨酸甲酯 [MAH] 含有聚（2-羟乙基甲基丙烯酸酯）[PHEMA] 的冷冻凝胶，以帮助增强细胞增殖。为了评估 MAH 共聚单体和明胶的效果，制备了含有不同量明胶和 MAH 的冷冻凝胶。因此，四组不同的冷冻凝胶是 PHEMA、聚（甲基丙烯酸 2-羟乙酯）-明胶 [PHEMA/GEL]、聚（甲基丙烯酸 2-羟乙酯-N-甲基丙烯酰基-（L）-组氨酸甲酯）[PHEMAH] 和聚(甲基丙烯酸2-羟乙酯-N-制备了甲基丙烯酰-(L)-组氨酸甲酯)-明胶 [PHEMAH/GEL]，根据不同的明胶比例，总共获得了八个冷冻凝胶组。进行了膨胀测试、扫描电子显微镜 (SEM)、显微计算机断层扫描 (Micro-CT) 和傅里叶变换红外光谱 - 衰减全反射 (FTIR-ATR) 以进行表征研究。FTIR-ATR 研究证实，明胶和 MAH 被掺入到冷冻凝胶结构中。Micro-CT 和 SEM 分析证实冷冻凝胶的平均孔径在 18-198 μm 范围内。然后，通过 MTT 分析对 L929 小鼠成纤维细胞中的细胞活力进行 24 小时和 48 小时的冷冻凝胶细胞增殖能力评估。PHEMAH/0.2GEL 冷冻凝胶的细胞活力达到 209%。