Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication,Advanced Science

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Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication
Advanced Science ( IF 14.3 ) Pub Date : 2021-08-02 , DOI: 10.1002/advs.202100825
Siyuan Ye _{1,

2,

3} , Wenzhe Li _{1,

4} , Huayi Wang _{1,

2,

5} , Ling Zhu _{1,

2} , Chen Wang _{1,

2} , Yanlian Yang _{1,

2}

Affiliation

The nanomechanical properties of tumor-derived small extracellular vesicles (sEVs) are essential to cancer progression. Here, nanoindentation is utilized on atomic force microscopy (AFM) to quantitatively investigate the nanomechanical properties of human breast cancer cell-derived sEVs at single vesicle level and explore their relationship with tumor malignancy and vesicle size. It is demonstrated that the stiffness of the sEVs results from the combined contribution of the bending modulus and osmotic pressure of the sEVs. The stiffness and osmotic pressure increase with increasing malignancy of the sEVs and decrease with increasing size of the sEVs. The bending modulus decreases with increasing malignancy of the sEVs and is lower in smaller sEVs. This study builds relationship between the nanomechanical signature of the sEV and tumor malignancy, adding information for better understanding cancer mechanobiology.

中文翻译：

用于肿瘤恶性肿瘤指示的小细胞外囊泡的定量纳米力学分析

肿瘤来源的小细胞外囊泡 (sEV) 的纳米力学特性对于癌症进展至关重要。在这里，利用原子力显微镜 (AFM) 上的纳米压痕在单个囊泡水平上定量研究人类乳腺癌细胞衍生的 sEV 的纳米力学特性，并探索它们与肿瘤恶性肿瘤和囊泡大小的关系。结果表明，sEV 的刚度是由 sEV 的弯曲模量和渗透压共同作用的结果。硬度和渗透压随着 sEV 恶性程度的增加而增加，并随着 sEV 大小的增加而降低。弯曲模量随着 sEV 恶性程度的增加而降低，并且在较小的 sEV 中弯曲模量较低。这项研究建立了 sEV 的纳米力学特征与肿瘤恶性肿瘤之间的关系，为更好地理解癌症力学生物学添加了信息。

更新日期：2021-09-22

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