Abstract

The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work. Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X_A) dosage of cellulase, (X_B) extraction time, (X_C) ultrasonic power, and (X_D) ratio of water to the material. The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM. THP was consisted of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, while glucose was the dominant (26.749 ± 0.634%). According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior. The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.

摘要

本工作研究了超声波酶法提取半枝莲多糖（THP）的优化及其单糖组成和抗氧化活性。采用单因素实验和响应面法（RSM）优化提取条件，自变量为（X _A）纤维素酶用量、（X _B）提取时间、（X _C）超声功率和（X _D) 水与材料的比例。在最佳条件下，THP的提取率有效提高，最大值（4.692±0.059%）与RSM的预测值非常吻合。THP由甘露糖、葡萄糖醛酸、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成，而葡萄糖占优势（26.749±0.634%）。根据 FRAP 法、DPPH 和羟基自由基清除法的总抗氧化能力测定，THP 显示出很强的抗氧化活性，具有剂量依赖性。结果表明，THP具有成为新型抗氧化剂的潜力，可以扩大其在食品和医药中的应用。