ABSTRACT

We developed a computer-assisted platform using laser scanning confocal microscopy to 3D reconstruct in real-time interactions between metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs). We demonstrate that MB-231 cancer cells migrate toward HUVEC networks, facilitated by filopodia, migrate along the network surfaces, penetrate into and migrate within the HUVEC networks, exit and continue migrating along network surfaces. The system is highly amenable to 3D reconstruction and computational analyses, and assessments of the effects of potential anti-metastasis monoclonal antibodies and other drugs. We demonstrate that an anti-RHAMM antibody blocks filopodium formation and all of the behaviors that we found take place between MB-231 cells and HUVEC networks.

摘要

我们开发了一个计算机辅助平台，使用激光扫描共聚焦显微镜对转移性乳腺癌细胞和人脐静脉内皮细胞 (HUVEC) 之间的实时相互作用进行 3D 重建。我们证明 MB-231 癌细胞在丝状伪足的促进下向 HUVEC 网络迁移，沿网络表面迁移，渗透到 HUVEC 网络中并在其中迁移，退出并继续沿网络表面迁移。该系统非常适合 3D 重建和计算分析，以及评估潜在的抗转移单克隆抗体和其他药物的效果。我们证明了一种抗 RHAMM 抗体阻断了丝状体的形成，并且我们发现的所有行为都发生在 MB-231 细胞和 HUVEC 网络之间。