In this study, bioaugmentation and biostimulation were used to explore the efficiency of bioremediation of hexachlorocyclohexane (HCH)-contaminated soil in the laboratory. A complex microbial community (germ A, abbreviated as A) was used for bioaugmentation. The KNO₃ and K₂HPO₄ (NP), wheat straw (JG), and glucose (PU) were used as exogenous amendments for biostimulation. Five treatments were set up: the control (CK), NP, A + NP, A + NP + JG, and A + NP + PU. After 28 days of remediation, the removal rate of total HCH (HCHs) in NP, A + NP, A + NP + JG, and A + NP + PU was 27.0%, 32.2%, 43.4%, and 45.3%, respectively. The results indicated that the removal of HCHs in A + NP, A + NP + JG, and A + NP + PU was higher than NP, indicating that bioaugmentation combined with biostimulation was more effective than biostimulation. Furthermore, compared with A + NP, A + NP + JG and A + NP + PU were more conducive to the degradation of HCHs. Because applying inorganic and organic nutrient substances simultaneously can effectively improve the living environment of microorganisms and increase microbial quantities in HCH-contaminated soil, therefore, the enzymatic reaction of microorganisms is accelerated, resulting in the efficiency of HCHs degradation improvement. The results indicated that bioaugmentation with germ A combine with biostimulation by organic and inorganic nutrients simultaneously constitutes a promising method for restoring soils contaminated with HCHs. This study would provide the basis for improving the bioremediation efficiency of HCH-contaminated soil.

在这项研究中，生物强化和生物刺激被用于探索实验室生物修复六氯环己烷 (HCH) 污染土壤的效率。一个复杂的微生物群落（germ A，缩写为 A）用于生物强化。KNO ₃和 K ₂ HPO ₄(NP)、麦秆 (JG) 和葡萄糖 (PU) 用作生物刺激的外源性改良剂。设置五个处理：对照（​​CK）、NP、A+NP、A+NP+JG和A+NP+PU。经过28天的修复，NP、A+NP、A+NP+JG、A+NP+PU中总HCHs的去除率分别为27.0%、32.2%、43.4%和45.3%。结果表明，A+NP、A+NP+JG、A+NP+PU对HCHs的去除率高于NP，表明生物强化联合生物刺激比生物刺激更有效。此外，与A+NP相比，A+NP+JG和A+NP+PU更有利于HCHs的降解。由于同时施用无机和有机营养物质可以有效改善微生物的生存环境，增加六氯环己烷污染土壤中微生物的数量，因此加速了微生物的酶促反应，从而提高了六氯环己烷的降解效率。结果表明，细菌 A 的生物强化与有机和无机营养物质的生物刺激相结合，构成了修复受六氯环己烷污染的土壤的有前景的方法。该研究将为提高六氯环己烷污染土壤的生物修复效率提供依据。结果表明，细菌 A 的生物强化与有机和无机营养物质的生物刺激相结合，构成了修复受六氯环己烷污染的土壤的有前景的方法。该研究将为提高六氯环己烷污染土壤的生物修复效率提供依据。结果表明，细菌 A 的生物强化与有机和无机营养物质的生物刺激相结合，构成了修复受六氯环己烷污染的土壤的有前景的方法。该研究将为提高六氯环己烷污染土壤的生物修复效率提供依据。