Pooled follicular fluid is easily accessible during ovum pick-up (OPU) and can be a potential source of non-invasive biochemical predictors for oocyte competence. Using the OPU procedure, oocytes recovered from a total of 60 Holstein heifers were used in in vitro embryo production. Pooled follicular fluid was collected at the time of OPU in order to establish a relationship between follicular biochemical components and the formation of blastocyst. The oocytes from each heifer were pooled together, matured in in vitro maturation medium for 24 h and then fertilized with sperm cells. The resulting zygotes were cultured for 7 days and the formation of blastocyst was recorded. Next, heifers were classified on the basis of the blastocyst formation, cleaved heifers and failed heifers. Follicular glucose concentrations were higher and the amount of C-reactive protein (CRP) was lower in cleaved heifers than in failed heifers. Moreover, the receiver operator characteristic (ROC) curve, clustering and network analysis showed the closeness between CRP, total bilirubin, and blastocyst yield. In conclusion, the findings imply that CRP follicular concentrations, which have been linked to heifer blastocyst formation, should be investigated further.

在取卵 (OPU) 期间，汇集的卵泡液很容易获得，并且可以成为非侵入性生化预测卵母细胞能力的潜在来源。使用 OPU 程序，从总共 60 头荷斯坦小母牛中回收的卵母细胞用于体外胚胎生产。在 OPU 时收集汇集的卵泡液，以建立卵泡生化成分与囊胚形成之间的关系。每头小母牛的卵母细胞汇集在一起​​，在体外成熟成熟培养基培养 24 小时，然后用精子细胞受精。将所得受精卵培养7天并记录胚泡的形成。接下来，小母牛根据囊胚形成、劈裂的小母牛和失败的小母牛进行分类。与失败的小母牛相比，被切割的小母牛的卵泡葡萄糖浓度更高，C 反应蛋白 (CRP) 的量更低。此外，受试者操作特征 (ROC) 曲线、聚类和网络分析显示 CRP、总胆红素和囊胚产量之间的接近程度。总之，这些发现意味着应该进一步研究与小母牛胚泡形成有关的 CRP 卵泡浓度。