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Transcriptome data-based identification of candidate genes involved in metabolism and accumulation of soluble sugars during fruit development in ‘Huangguan’ plum
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-02 , DOI: 10.1111/jfbc.13878 Xinmiao Yu 1 , Muhammad Moaaz Ali 1 , Binqi Li 1, 2 , Ting Fang 1 , Faxing Chen 1, 2
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-02 , DOI: 10.1111/jfbc.13878 Xinmiao Yu 1 , Muhammad Moaaz Ali 1 , Binqi Li 1, 2 , Ting Fang 1 , Faxing Chen 1, 2
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Fruit sweetness being an important factor of organoleptic quality directly affects the consumers' preferences for fresh fruit consumption, and is influenced by the composition and quantity of sugars. In this study, four soluble sugars (sucrose, fructose, glucose, and sorbitol) were identified and quantified in plum fruits cv. ‘Huangguan’ at four different maturity stages (fruitlet, green, veraison, and mature stage). The results revealed that sucrose and glucose are major soluble sugar components at the fruitlet and mature stages, respectively. RNA-Seq analysis was carried out and 6,778 differentially expressed genes (DEGs) were identified, including 121 genes involved in sugar metabolism. Furthermore, a total of 39 transcripts of 8 gene families encoding key enzymes related to the metabolism and accumulation of soluble sugars were separately identified. ERD6L (gene 103322904) was involved in keeping a balance of glucose between the inside and outside of vacuole. SS (gene 103333990) and SDH (gene 103335104) regulated the accumulation of fructose at the green stage. SDH (gene 103335104) controlled the degradation of sorbitol at the green stage. SS (gene 103333990) and PFK (gene 103333391) regulated the degradation of sucrose at the early stages of fruit development. Moreover, NINV (gene 103331108) regulated the accumulation of total sugar in plum. Genes 103321334 and 103335689 were important bZIP transcription factors that regulate the accumulation of glucose and fructose in fruits. Twelve DEGs were selected and validated to observe the relative accuracy of transcriptome sequencing data using qRT-PCR. Gene expression patterns were consistent between qRT-PCR and RNA-Seq data, indicating the reliability of RNA-Seq data.
中文翻译：
基于转录组数据的‘黄冠’李果实发育过程中参与可溶性糖代谢和积累的候选基因鉴定
水果甜度作为感官品质的重要因素，直接影响消费者对新鲜水果消费的偏好，并受糖分的组成和数量的影响。在这项研究中，四种可溶性糖（蔗糖、果糖、葡萄糖和山梨糖醇）在李子果实 cv. 中被鉴定和量化。'黄冠'在四个不同的成熟阶段（小果、绿色、veraison和成熟阶段）。结果表明，蔗糖和葡萄糖分别是果实和成熟阶段的主要可溶性糖成分。进行了 RNA-Seq 分析，鉴定了 6,778 个差异表达基因 (DEG)，其中包括 121 个参与糖代谢的基因。此外，分别鉴定了编码与可溶性糖代谢和积累相关的关键酶的8个基因家族的39个转录本。ERD6L（基因 103322904）参与保持液泡内外葡萄糖的平衡。SS（基因 103333990）和 SDH（基因 103335104）调节果糖在绿色阶段的积累。SDH（基因103335104）在绿色阶段控制山梨糖醇的降解。SS（基因 103333990）和 PFK（基因 103333391）在果实发育的早期调节蔗糖的降解。此外，NINV（基因103331108）调节了李子中总糖的积累。基因 103321334 和 103335689 是重要的 bZIP 转录因子，可调节水果中葡萄糖和果糖的积累。选择并验证了 12 个 DEG，以使用 qRT-PCR 观察转录组测序数据的相对准确性。qRT-PCR 和 RNA-Seq 数据之间的基因表达模式一致，表明 RNA-Seq 数据的可靠性。
更新日期：2021-09-06
中文翻译：
基于转录组数据的‘黄冠’李果实发育过程中参与可溶性糖代谢和积累的候选基因鉴定
水果甜度作为感官品质的重要因素，直接影响消费者对新鲜水果消费的偏好，并受糖分的组成和数量的影响。在这项研究中，四种可溶性糖（蔗糖、果糖、葡萄糖和山梨糖醇）在李子果实 cv. 中被鉴定和量化。'黄冠'在四个不同的成熟阶段（小果、绿色、veraison和成熟阶段）。结果表明，蔗糖和葡萄糖分别是果实和成熟阶段的主要可溶性糖成分。进行了 RNA-Seq 分析，鉴定了 6,778 个差异表达基因 (DEG)，其中包括 121 个参与糖代谢的基因。此外，分别鉴定了编码与可溶性糖代谢和积累相关的关键酶的8个基因家族的39个转录本。ERD6L（基因 103322904）参与保持液泡内外葡萄糖的平衡。SS（基因 103333990）和 SDH（基因 103335104）调节果糖在绿色阶段的积累。SDH（基因103335104）在绿色阶段控制山梨糖醇的降解。SS（基因 103333990）和 PFK（基因 103333391）在果实发育的早期调节蔗糖的降解。此外，NINV（基因103331108）调节了李子中总糖的积累。基因 103321334 和 103335689 是重要的 bZIP 转录因子，可调节水果中葡萄糖和果糖的积累。选择并验证了 12 个 DEG，以使用 qRT-PCR 观察转录组测序数据的相对准确性。qRT-PCR 和 RNA-Seq 数据之间的基因表达模式一致，表明 RNA-Seq 数据的可靠性。
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