Background

Overexpression of the abiotic and biotic stress-resistance genes of the plant signaling pathway is well known for its significant role in the regulation of plant growth and enhancement of the productivity of agricultural land under changing climatic conditions.

Objectives

This research aimed to clone Populus davidiana × Populus bolleana PP2C (PdPP2C) gene and analyze its structure and function, and downregulate PdPP2C by overexpression of its antisense PdPP2C (AS-PdPP2C) gene for enhancing cold resistance in transgenic lines of hybrid P. davidiana × P. bolleana.

Methods

PdPP2C was cloned and transformed to identify its function, and its antisense was overexpressed via downregulation to increase the cold resistance in transgenic lines of hybrid P. davidiana × P. bolleana.

Results

Antisense inhibition of protein phosphatase 2C accelerates the cold acclimation of Poplar (P. davidiana × P. bolleana) gene in terms of antifreeze.

Conclusion

PdPP2C was expressed in the roots, stems, and leaves of P. davidiana × P. bolleana, and the expression was higher in the leaves. The expression of PdPP2C was also significantly downregulated at low-temperature (0 °C and 4 °C) stress. The relative conductivity and malondialdehyde content of non-transgenic lines were higher than those of AS-PdPP2C lines after 2 days of cold treatment at − 1 °C. The leaves of the transgenic lines were not wilted and showed no chlorosis compared with those of the non-transgenic lines. The AS-PdPP2C transgenic lines also showed higher freezing resistance than the non-transgenic lines. AS-PdPP2C participated in the regulation of freezing resistance.

中文翻译：

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反义磷酸酶 2C 的过表达提供了杂交杨树 × 波罗杨的耐寒性

背景

植物信号通路的非生物和生物胁迫抗性基因的过度表达因其在气候条件变化下调节植物生长和提高农田生产力的重要作用而广为人知。

目标

本研究旨在克隆Populus davidiana  ×  Populus bolleana PP2C ( PdPP2C ) 基因并分析其结构和功能，并通过过表达其反义PdPP2C ( AS-PdPP2C ) 基因下调PdPP2C以增强杂交P. davidiana  × 转基因品系的抗寒性。P. bolleana。

方法

克隆并转化PdPP2C以鉴定其功能，并通过下调其反义基因来增加杂交P. davidiana  ×  P. bolleana转基因品系的耐寒性。

结果

蛋白磷酸酶2C的反义抑制加速了杨树（P. davidiana  ×  P. bolleana）基因在抗冻方面的冷驯化。

结论

PdPP2C在P. davidiana  ×  P. bolleana的根、茎和叶中均有表达，在叶中表达量较高。PdPP2C的表达在低温（0°C和4°C）胁迫下也显着下调。在-1℃冷处理2天后，非转基因品系的相对电导率和丙二醛含量高于AS-PdPP2C品系。与非转基因品系相比，转基因品系的叶子没有枯萎并且没有表现出萎黄病。AS-PdPP2C转基因株系也表现出比非转基因株系更高的抗冻性。AS-PdPP2C参与了抗冻性的调节。