Abstract

The pufferfish are non-indigenous species in the Mediterranean Sea that do not have predators in the food chain. Therefore, the determination of heavy metal accumulation in pufferfish species can be considered as natural chelation for the aquatic environment. The present study, the potential usage of dentin tissue as an indicator of heavy metal bioaccumulation was examined in pufferfish species. The accumulation of Co, Cu, Zn, Fe, Mo, Ni, Se, Al, Cd, Pb, As, Cr in dentin, liver, kidney and muscle tissues of four pufferfish species (Lagocephalus sceleratus, Lagocephalus spadiceus, Lagocephalus suezensis, and Torquigener flavimaculosus) were captured in the Göksu River and Yeşjlovacık coasts of Turkey by a commercial trawler. The different bioaccumulation levels were found in each species in terms of metals concentrations and tissues. The metal levels in dentine tissue were found to be similar to the brain. Aluminium had the highest accumulation among these tissues. The liver and gill tissues showed the same bioaccumulation properties with each other. Zn had the highest accumulation among these tissues (P < 0.05). The muscle was different from metabolically active tissues in terms of metal accumulation that arsenic had the highest bioaccumulation (P < 0.05). Fe, Se, As, Zn, Cu, Cr and Al were accumulated in all tissue types, however, only Se was not detected in dentin tissue. On the other hand, Co, Ni, Mo, Cd and Pb were not detected in any tissue types. The relative accumulation of metals in dentin tissue was determined as Al > Zn > Cr > Fe > As > Cu. The present study for the first time revealed that dentin tissue can be used as an indicator tissue for toxicology studies that metal accumulations in dentin were determined in parallel with other examined tissues of pufferfish species.

中文翻译：

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河豚牙本质作为金属积累指示剂的潜在用途

摘要

河豚是地中海的非本土物种，在食物链中没有捕食者。因此，测定河豚种类中重金属的积累可以被认为是对水生环境的自然螯合。本研究在河豚物种中检查了牙本质组织作为重金属生物积累指标的潜在用途。Co、Cu、Zn、Fe、Mo、Ni、Se、Al、Cd、Pb、As、Cr在四种河豚（Lagocephalus sceleratus、Lagocephalus spadiceus、Lagocephalus suezensis和黄斑龟) 被一艘商业拖网渔船在土耳其的 Göksu 河和 Yeşjlovacık 海岸捕获。就金属浓度和组织而言，在每个物种中发现了不同的生物积累水平。发现牙本质组织中的金属水平与大脑相似。在这些组织中，铝的积累量最高。肝脏和鳃组织彼此显示出相同的生物蓄积特性。Zn 在这些组织中的积累量最高（P < 0.05）。肌肉在金属积累方面不同于代谢活跃的组织，砷的生物积累量最高（P< 0.05)。Fe、Se、As、Zn、Cu、Cr 和 Al 在所有组织类型中都有积累，但在牙本质组织中仅检测到 Se。另一方面，在任何组织类型中均未检测到 Co、Ni、Mo、Cd 和 Pb。金属在牙本质组织中的相对积累被确定为 Al > Zn > Cr > Fe > As > Cu。本研究首次揭示牙本质组织可用作毒理学研究的指示组织，即牙本质中的金属积累与河豚的其他检查组织同时测定。