Abstract

The deficiency of essential minerals increases lead absorption and thus aggravates the lead-induced toxic effects. This study was aimed at understanding the ameliorative effect of essential minerals on lead-induced alterations in hematological parameters in rats. To achieve this objective, the study was conducted in 320 male Wistar albino rats, grouped into two, with equal numbers. One of the groups of rats was fed on a mineral-supplemented food referred to as a ‘well-nourished group’ and another group ‘undernourished group’ on food without mineral supplements. Each group of rats was further subdivided into ‘Subjects’ and ‘Controls.’ Subjects of both the groups of rats were exposed to 500 ppm lead acetate up to a period of 300 days (10 months) in drinking water and the role of minerals on lead-induced alterations in hematological parameters was evaluated. A significant decrease (p < 0.001) in hemoglobin (Hb) and δ-aminolevulinic acid dehydratase (δ-ALAD) levels and a significant increase (p < 0.001) in urinary δ-aminolevulinic acid (δ-ALAU) levels were seen in subjects without mineral supplementation compared to those fed on a mineral-enriched diet. A positive correlation was observed between blood lead levels (PbB) and δ-ALAU (r = 0.792) and a negative correlation with Hb (r = −0.926) and δ-ALAD (r = −0.836) in the subjects. These changes were very prominent in the undernourished subjects when compared to the well-nourished subjects. Observations of the present study indicate that mineral supplementation with ongoing lead exposure may help in minimizing the absorption of lead and reduce lead-induced toxic effects.

摘要

必需矿物质的缺乏会增加铅的吸收，从而加剧铅引起的毒性作用。本研究旨在了解必需矿物质对铅引起的大鼠血液学参数改变的改善作用。为了实现这一目标，该研究在 320 只雄性 Wistar 白化病大鼠中进行，分为两组，数量相等。其中一组大鼠以补充矿物质的食物喂养，称为“营养良好组”，另一组“营养不良组”以不含矿物质补充剂的食物喂养。每组大鼠进一步细分为“受试者”和“对照”。' 两组大鼠的受试者在饮用水中暴露于 500 ppm 醋酸铅长达 300 天（10 个月），并评估了矿物质对铅引起的血液学参数改变的作用。显着下降（ 与未补充矿物质的受试者相比，血红蛋白 (Hb) 和 δ-氨基乙酰丙酸脱水酶 (δ-ALAD) 水平的 p < 0.001) 和尿 δ-氨基乙酰丙酸 (δ-ALAU) 水平的显着增加 ( p  < 0.001 )对于那些以富含矿物质的饮食为食的人。血铅水平 (PbB) 与 δ-ALAU ( r  = 0.792) 呈正相关，与 Hb ( r = -0.926) 和 δ-ALAD ( r= -0.836）在受试者中。与营养良好的受试者相比，这些变化在营养不良的受试者中非常突出。本研究的观察结果表明，在持续接触铅的情况下补充矿物质可能有助于最大限度地减少铅的吸收并减少铅引起的毒性作用。