Lipases are the versatile biological catalysts used in a wide range of commercial synthetic applications. Microbial lipases have been widely used in the pharmaceutical, food, textile, oleochemical, paper, and bioenergy industries, in the composition of detergents, and in the production of polymers and enantiomerically pure chemical intermediates. Lipases have a unique lid opening mechanism that makes them interesting biocatalysts to catalyze reactions in hydrophobic environments. Water insoluble substrates have also been efficiently transformed by lipases in organic solvents. The use of lipases in industrial processes could be limited by the cost of their production, the difficulties in their recovery and disposal, or by the harsh conditions of some processes that could inactivate or denature the protein. The immobilization of enzymes helps overcome these physical and economical drawbacks, making the catalyst more active, stable, and reusable, resulting in the reduction of costs and of contaminating and harmful byproducts. Polypropylene has unique properties that aid in the activation of the lid opening mechanism and improve the catalytic efficiency of immobilized lipases. The aim of this review is to present an overview of the uses of polypropylene as a support for immobilization of lipolytic enzymes, the study of the immobilization procedure conditions, the characteristics of the immobilized enzymes, and the perspectives of their use in the future as a powerful tool in sustainable industry. © 2021 Society of Chemical Industry (SCI).

中文翻译：

---

聚丙烯作为脂解酶固定化的选择性载体：超活化、纯化和生物技术应用

脂肪酶是广泛用于商业合成应用的多功能生物催化剂。微生物脂肪酶已广泛用于制药、食品、纺织、油脂化工、造纸和生物能源行业、洗涤剂组合物以及聚合物和对映体纯化学中间体的生产。脂肪酶具有独特的开盖机制，使其成为在疏水环境中催化反应的有趣生物催化剂。水不溶性底物也已被有机溶剂中的脂肪酶有效转化。脂肪酶在工业过程中的使用可能会受到其生产成本、其回收和处置的困难或某些可能使蛋白质失活或变性的过程的恶劣条件的限制。酶的固定化有助于克服这些物理上和经济上的缺点，使催化剂更加活跃、稳定和可重复使用，从而降低成本，减少污染和有害副产物。聚丙烯具有独特的特性，有助于激活盖子打开机制并提高固定化脂肪酶的催化效率。本综述的目的是概述聚丙烯作为脂肪分解酶固定化载体的用途、固定化程序条件的研究、固定化酶的特性以及它们在未来用作脂肪分解酶的前景。可持续产业的强大工具。© 2021 化学工业协会 (SCI)。从而降低成本以及减少污染和有害的副产品。聚丙烯具有独特的特性，有助于激活盖子打开机制并提高固定化脂肪酶的催化效率。本综述的目的是概述聚丙烯作为脂肪分解酶固定化载体的用途、固定化程序条件的研究、固定化酶的特性以及它们在未来用作脂肪分解酶的前景。可持续产业的强大工具。© 2021 化学工业协会 (SCI)。从而降低成本以及减少污染和有害的副产品。聚丙烯具有独特的特性，有助于激活盖子打开机制并提高固定化脂肪酶的催化效率。本综述的目的是概述聚丙烯作为脂肪分解酶固定化载体的用途、固定化程序条件的研究、固定化酶的特性以及它们在未来用作脂肪分解酶的前景。可持续产业的强大工具。© 2021 化学工业协会 (SCI)。本综述的目的是概述聚丙烯作为脂肪分解酶固定化载体的用途、固定化程序条件的研究、固定化酶的特性以及它们在未来用作脂肪分解酶的前景。可持续产业的强大工具。© 2021 化学工业协会 (SCI)。本综述的目的是概述聚丙烯作为脂肪分解酶固定化载体的用途、固定化程序条件的研究、固定化酶的特性以及它们在未来用作脂肪分解酶的前景。可持续产业的强大工具。© 2021 化学工业协会 (SCI)。