Taenia solium oncosphere protein TSOL18 is the host-protective antigen against porcine cysticercosis. Little attention has been given to use it as target molecule in immunodiagnostic tests. The objective of this paper is to describe the immunodiagnostic potential of monoclonal antibodies (MoAbs) raised against conformational epitopes of TSOL18. Three murine IgG1 MoAbs (25D12C1, 21C2D2, 10H1F2) against three different conformational epitopes of TSOL18 were produced and evaluated with an inhibition enzyme-linked immunosorbent assay (i-ELISA) for the detection of anti-TSOL18 and anti-oncosphere antibodies. Serum samples from pigs immunized with TSOL18 inhibited the binding of the three MoAbs to TSOL18 antigen in i-ELISA. The highest inhibition of anti-TSOL18 antibodies in immunized pigs was observed with MoAb 25D12C1. Ten field sera (12.19%) from 82 non-vaccinated and non-infected pigs showed anti-oncosphere antibodies inhibiting the binding of MoAb 25D12C1. Anti-oncosphere antibodies in pigs experimentally infected with T. solium eggs inhibited the binding of MoAb 25D12C1 from 2 to 8 week-post infection. It is concluded that MoAb 25D12C1 has excellent immunodiagnostic potentials to detect anti-oncosphere antibodies in the intermediate hosts at early exposure to T. solium eggs. Further investigations on potential use of MoAb 25D12C1 in a capture antigen ELISA for the detection of post-oncospheral antigens in infected pigs cannot be overemphasized.

猪带绦虫Oncosphere 蛋白 TSOL18 是针对猪囊尾蚴病的宿主保护性抗原。很少有人注意将其用作免疫诊断测试中的靶分子。本文的目的是描述针对 TSOL18 构象表位产生的单克隆抗体 (MoAb) 的免疫诊断潜力。产生了针对 TSOL18 的三种不同构象表位的三种鼠 IgG1 MoAb（25D12C1、21C2D2、10H1F2），并使用抑制酶联免疫吸附试验 (i-ELISA) 进行评估，以检测抗 TSOL18 和抗电球抗体。在 i-ELISA 中，来自用 TSOL18 免疫的猪的血清样本抑制了三种 MoAb 与 TSOL18 抗原的结合。使用 MoAb 25D12C1 观察到免疫猪中抗 TSOL18 抗体的最高抑制。十场血清 (12. 19%) 来自 82 只未接种疫苗和未感染的猪显示出抗肿瘤抗体抑制 MoAb 25D12C1 的结合。实验感染猪的抗肿瘤抗体猪带绦虫卵抑制的MoAb 25D12C1的2至8周后感染的结合。结论的MoAb 25D12C1具有优异的免疫诊断电位，以检测早期暴露于中间宿主抗六钩蚴抗体猪带绦虫卵。进一步研究 MoAb 25D12C1 在捕获抗原 ELISA 中的潜在用途，以检测感染猪的肿瘤后抗原，怎么强调都不过分。