Understanding the interplay between immune and structural cells is important for studying fibrosis and inflammation; however, primary immune cell isolation from organs that are typically enriched in stromal cells, like the lung, esophagus, or gut, proves to be an ongoing challenge. In fibrotic conditions, this challenge becomes even greater as infiltrating cells become trapped in the robust extracellular matrix (ECM). This protocol details a method to isolate cells at high yield from stroma-rich organs that can be used for further analyses via flow cytometry, stimulation, or culturing. Validation of this method is confirmed by flow cytometry data assessing immune cell populations of interest. This protocol can be completed in approximately 5–6 h.

了解免疫细胞和结构细胞之间的相互作用对于研究纤维化和炎症很重要；然而，从通常富含基质细胞的器官（如肺、食道或肠道）中分离初级免疫细胞被证明是一个持续的挑战。在纤维化条件下，随着浸润细胞被困在坚固的细胞外基质（ECM）中，这一挑战变得更加严峻。该协议详细介绍了一种从富含基质的器官中高产量分离细胞的方法，该方法可用于通过流式细胞术、刺激或培养进行进一步分析。通过评估感兴趣的免疫细胞群的流式细胞术数据证实了该方法的有效性。该协议可在大约 5-6 小时内完成。