Background

Osteoarthritis (OA) has become the most common type of arthritis, and the cellular and molecular mechanisms involved in OA is complex and not fully understood. Inflammation and matrix degradation have been indicated to play vital roles in pathogenesis of OA. Apart from biochemical molecules such as anti-oxidants which have been suggested to reduce inflammatory responses, photobiomodulation has become interesting targets for developing therapeutic strategies of cartilage repair and regeneration.

Methods

To investigate the effect of photobiomodulation on OA, red light-emitting diode (LED) was preventatively applied to hydrogen peroxide induced OA-like chondrocytes. Lumen density was measured for the level of free radicals and gene expression of IL-1β, TNF-α, MMP-1, MMP-13, and COL-2 was analyzed.

Significant Findings

Our results showed that pre-treatment of 635-nm LED for 2 h significantly reduced free radical formation (from 100% to 8.25%) and inflammatory gene IL-1β, TNF-α expression in OA-like cells while matrix gene COL-2 was enhanced. Furthermore, combination of preventative LED treatment with anti-oxidant(s) (vitamin E or/and resveratrol) exerted synergistic effects of anti-inflammation and MMP-13 reduction. In conclusion, preventative LED irradiation combined with anti-oxidants provides a novel therapeutic strategy for treating early OA prior to the progression of the disease.

中文翻译：

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红色发光二极管的预防性治疗保护骨关节炎样软骨细胞免受氧化应激诱导的炎症并促进基质基因表达

背景

骨关节炎 (OA) 已成为最常见的关节炎类型，OA 涉及的细胞和分子机制复杂且尚未完全了解。炎症和基质降解已被证明在 OA 的发病机制中起着至关重要的作用。除了已被建议减少炎症反应的抗氧化剂等生化分子外，光生物调节已成为开发软骨修复和再生治疗策略的有趣目标。

方法

为了研究光生物调节对 OA 的影响，将红色发光二极管 (LED) 预防性地应用于过氧化氢诱导的 OA 样软骨细胞。测量管腔密度的自由基水平，分析 IL-1β、TNF-α、MMP-1、MMP-13 和 COL-2 的基因表达。

重要发现

我们的结果表明，635 nm LED 预处理 2 h 显着降低了 OA 样细胞中自由基的形成（从 100% 到 8.25%）和炎症基因 IL-1β、TNF-α 的表达，而基质基因 COL-2得到了增强。此外，预防性 LED 治疗与抗氧化剂（维生素 E 或/和白藜芦醇）的组合发挥了抗炎和 MMP-13 减少的协同作用。总之，预防性 LED 照射与抗氧化剂相结合，为在疾病进展之前治疗早期 OA 提供了一种新的治疗策略。