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Linear triglycerol-based fluorosurfactants show high potential for droplet-microfluidics-based biochemical assays
Soft Matter ( IF 2.9 ) Pub Date : 2021-08-02 , DOI: 10.1039/d1sm00890k
Mohammad Suman Chowdhury 1 , Wenshan Zheng 2 , Abhishek Kumar Singh 1 , Irvine Lian Hao Ong 3 , Yong Hou 1 , John A Heyman 4 , Abbas Faghani 1 , Esther Amstad 3 , David A Weitz 4 , Rainer Haag 1
Affiliation  

Fluorosurfactants have expanded the landscape of high-value biochemical assays in microfluidic droplets, but little is known about how the spatial geometries and polarity of the head group contribute to the performance of fluorosurfactants. To decouple this, we design, synthesize, and characterize two linear and two dendritic glycerol- or tris-based surfactants with a common perfluoropolyether tail. To reveal the influence of spatial geometry, we choose inter-droplet cargo transport as a stringent test case. Using surfactants with linear di- and triglycerol, we show that the inter-droplet cargo transport is minimal compared with their dendritic counterparts. When we encapsulated a less-leaky sodium fluorescent dye into the droplets, quantitatively, we find that the mean fluorescence intensity of the PFPE-dTG stabilized PBS-only droplets after 72 h was ∼3 times that of the signal detected in PBS-only droplets stabilized by PFPE-lTG. We also demonstrate that the post-functionalization of PFPE-lTG having a linear geometry and four hydroxy groups enables the ‘from-Droplet’ fishing of the biotin–streptavidin protein complex without the trade-off between fishing efficiency and droplet stability. Thus, our approach to design user-friendly surfactants reveals the aspects of spatial geometry and facile tunability of the polar head groups that have not been captured or exploited before.

中文翻译:

基于线性甘油三酯的含氟表面活性剂显示出基于液滴微流体的生化分析的巨大潜力

含氟表面活性剂扩大了微流体液滴中高价值生化分析的范围,但关于头部基团的空间几何形状和极性如何影响含氟表面活性剂的性能却知之甚少。为了解决这个问题,我们设计、合成并表征了两种具有共同全氟聚醚尾部的线性和两种树枝状甘油或三基表面活性剂。为了揭示空间几何的影响,我们选择液滴间货物运输作为严格的测试案例。使用具有线性二甘油和三甘油的表面活性剂,我们表明与树枝状对应物相比,液滴间的货物运输最小。当我们将泄漏较少的钠荧光染料封装到液滴中时,定量地,我们发现 PFPE-dTG 稳定的仅 PBS 液滴的平均荧光强度在 72 小时后是在 PFPE-lTG 稳定的仅 PBS 液滴中检测到的信号的约 3 倍。我们还证明,具有线性几何形状和四个羟基的 PFPE-lTG 的后功能化能够实现生物素-链霉亲和素蛋白复合物的“从液滴”捕捞,而无需在捕捞效率和液滴稳定性之间进行权衡。因此,我们设计用户友好型表面活性剂的方法揭示了以前未被捕获或利用的极性头部基团的空间几何形状和易于调节的方面。我们还证明,具有线性几何形状和四个羟基的 PFPE-lTG 的后功能化能够实现生物素-链霉亲和素蛋白复合物的“从液滴”捕捞,而无需在捕捞效率和液滴稳定性之间进行权衡。因此,我们设计用户友好型表面活性剂的方法揭示了以前未被捕获或利用的极性头部基团的空间几何形状和易于调节的方面。我们还证明,具有线性几何形状和四个羟基的 PFPE-lTG 的后功能化能够实现生物素-链霉亲和素蛋白复合物的“从液滴”捕捞,而无需在捕捞效率和液滴稳定性之间进行权衡。因此,我们设计用户友好型表面活性剂的方法揭示了以前未被捕获或利用的极性头部基团的空间几何形状和易于调节的方面。
更新日期:2021-08-02
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