Selective increment of phosphatidylserine on the autophagic body membrane in the yeast vacuole,FEBS Letters

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Selective increment of phosphatidylserine on the autophagic body membrane in the yeast vacuole
FEBS Letters ( IF 3.0 ) Pub Date : 2021-08-02 , DOI: 10.1002/1873-3468.14167
Yuki Yamakuchi ₁ , Yuna Kurokawa ₁ , Rikako Konishi ₁ , Kayoko Fukuda ₁ , Tatsunori Masatani ₂ , Akikazu Fujita ₁

Affiliation

In yeast cells, the autophagosome is a double-membrane structure; the inner membrane becomes the autophagic body membrane in the vacuole. Vacuolar enzymes degrade the autophagic body. There is no critical information regarding its selective degradation. Using the electron microscopy method, distributions of four phospholipids were examined in the autophagosomal and autophagic body membranes upon autophagy induction. The labeling of phosphatidylserine (PtdSer) in the autophagic body membrane dramatically increased after it converted from the autophagosome, but remained low in the vacuolar membrane. PtdSer in the autophagic body membrane also increased in atg15∆ yeast. These results suggest that the selective increment of PtdSer in the autophagic body, but not the vacuolar, membrane, can explain the selective degradation of the autophagic membrane.

中文翻译：

酵母液泡自噬体膜上磷脂酰丝氨酸的选择性增加

在酵母细胞中，自噬体是双膜结构；内膜成为液泡中的自噬体膜。液泡酶降解自噬体。没有关于其选择性降解的关键信息。使用电子显微镜方法，在自噬诱导后检查了四种磷脂在自噬体和自噬体膜中的分布。自噬体转化自自噬体后，自噬体膜中磷脂酰丝氨酸 (PtdSer) 的标记显着增加，但在液泡膜中的标记仍然很低。自噬体膜中的PtdSer在atg15 中也增加∆ 酵母。这些结果表明，自噬体而非液泡膜中 PtdSer 的选择性增加可以解释自噬膜的选择性降解。

更新日期：2021-09-13

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