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Synthesis, characterization, and evaluation of curcumin-loaded endodontic reparative material
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2021-07-31 , DOI: 10.1002/jbt.22854 Mahdieh Alipour 1, 2 , Sadaf Fadakar 3 , Marziyeh Aghazadeh 4 , Roya Salehi 5 , Hossein Samadi Kafil 6 , Leila Roshangar 2 , Ensieh Mousavi 3 , Zahra Aghazadeh 4
Journal of Biochemical and Molecular Toxicology ( IF 3.2 ) Pub Date : 2021-07-31 , DOI: 10.1002/jbt.22854 Mahdieh Alipour 1, 2 , Sadaf Fadakar 3 , Marziyeh Aghazadeh 4 , Roya Salehi 5 , Hossein Samadi Kafil 6 , Leila Roshangar 2 , Ensieh Mousavi 3 , Zahra Aghazadeh 4
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Curcumin (CUR) is an ancient therapeutic agent with remarkable antimicrobial and anti-inflammatory properties. The purpose of the current study was to synthesize and evaluate a curcumin-based reparative endodontic material to reduce infection and inflammation besides the induction of mineralization during the healing of the dentin–pulp complex. Poly-ɛ-caprolactone (PCL)/gelatin (Gel)/CUR scaffold was synthesized and assessed by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermo-gravimetric analysis (TGA). Agar diffusion test was performed against E. coli, A. baumannii, P. aeruginosa, S. aureus, E. faecalis, and S. mutans. Moreover, proliferative, antioxidative, anti-inflammatory, and calcification properties of these scaffolds on human dental pulp stem cells (hDPSCs) were evaluated. The results showed that PCL/Gel/CUR scaffold had antibacterial effects. Also, these CUR-based scaffolds had significant inhibitory effects on the expression of tumor necrosis factor α and DCF from inflamed hDPSCs (p < 0.05). Moreover, the induction of mineralization in hDPSCs significantly increased after seeding on CUR-based scaffolds (p < 0.05). Based on these findings, the investigated CUR-loaded material was fabricated successfully and provided an appropriate structure for the attachment and proliferation of hDPSCs. It was found that these scaffolds had antimicrobial, antioxidant, and anti-inflammatory characteristics and could induce mineralization in hDPSCs, which is essential for healing and repairing the injured dentin–pulp complex.
中文翻译：
姜黄素牙髓修复材料的合成、表征和评价
姜黄素 (CUR) 是一种古老的治疗剂，具有显着的抗菌和抗炎特性。本研究的目的是合成和评估一种基于姜黄素的修复性牙髓材料，除了在牙本质 - 牙髓复合体愈合过程中诱导矿化外，还可以减少感染和炎症。聚-ɛ-己内酯 (PCL)/明胶 (Gel)/CUR 支架合成并通过扫描电子显微镜、傅里叶变换红外光谱和热重分析 (TGA) 进行评估。针对大肠杆菌、鲍曼不动杆菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌和变形链球菌进行琼脂扩散试验. 此外，还评估了这些支架对人牙髓干细胞 (hDPSC) 的增殖、抗氧化、抗炎和钙化特性。结果表明PCL/Gel/CUR支架具有抗菌作用。此外，这些基于 CUR 的支架对来自发炎的 hDPSC 的肿瘤坏死因子 α 和 DCF 的表达具有显着的抑制作用（p < 0.05）。此外，在基于 CUR 的支架上接种后，hDPSCs 的矿化诱导显着增加（p < 0.05)。基于这些发现，所研究的 CUR 负载材料被成功制造，并为 hDPSCs 的附着和增殖提供了合适的结构。发现这些支架具有抗菌、抗氧化和抗炎特性，可以诱导 hDPSCs 的矿化，这对于愈合和修复受损的牙本质 - 牙髓复合体至关重要。
更新日期：2021-09-15
中文翻译：
姜黄素牙髓修复材料的合成、表征和评价
姜黄素 (CUR) 是一种古老的治疗剂，具有显着的抗菌和抗炎特性。本研究的目的是合成和评估一种基于姜黄素的修复性牙髓材料，除了在牙本质 - 牙髓复合体愈合过程中诱导矿化外，还可以减少感染和炎症。聚-ɛ-己内酯 (PCL)/明胶 (Gel)/CUR 支架合成并通过扫描电子显微镜、傅里叶变换红外光谱和热重分析 (TGA) 进行评估。针对大肠杆菌、鲍曼不动杆菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌和变形链球菌进行琼脂扩散试验. 此外，还评估了这些支架对人牙髓干细胞 (hDPSC) 的增殖、抗氧化、抗炎和钙化特性。结果表明PCL/Gel/CUR支架具有抗菌作用。此外，这些基于 CUR 的支架对来自发炎的 hDPSC 的肿瘤坏死因子 α 和 DCF 的表达具有显着的抑制作用（p < 0.05）。此外，在基于 CUR 的支架上接种后，hDPSCs 的矿化诱导显着增加（p < 0.05)。基于这些发现，所研究的 CUR 负载材料被成功制造，并为 hDPSCs 的附着和增殖提供了合适的结构。发现这些支架具有抗菌、抗氧化和抗炎特性，可以诱导 hDPSCs 的矿化，这对于愈合和修复受损的牙本质 - 牙髓复合体至关重要。
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