The immobilization of Horseradish Peroxidase enzyme over Magnesium (Mg-nHT) and Nickel (Ni-nHT) nano Hydrotalcite clays were synthesized by the co-precipitation method, and later modified with gold nanoparticles through in-situ synthesis and delamination-restructuration, is reported. The synthesized materials were characterized by Fourier-Transform Infrared Spectroscopy, X-ray diffraction, Scanning Electronic Microscopy, Flame Atomic Absorption Spectroscopy and Cyclic Voltammetry. Results show that the size of the synthetized materials is 26 nm for the gold nanoparticles, and 38.92 nm and 8.44 nm for Mg-AlnHT and Ni-AlnHT, respectively. The clays were used to modify the surface of graphite electrodes (GE); the inclusion of Au nanoparticles in the Hydrotalcites structure improved the enzyme-electrode electric communication. Figures of merit for detection of H₂O₂ by Differential-Pulse Voltammetry at HRP/Mg-AlnHT/GE, HRP/Ni-AlnHT/GE, HRP-AuNps/Mg-AlnHT/GE and HRP-AuNps/Ni-AlnHT/GE modified electrodes were determined, and respective detection limits of 0.012 μmolL⁻¹, 0.014 μmolL⁻¹, 0.009 μmolL⁻¹, and 0.010 μmolL^{− 1} were obtained.

通过共沉淀法合成了辣根过氧化物酶固定在镁（Mg-nHT）和镍（Ni-nHT）纳米水滑石粘土上，然后通过原位合成和分层重组用金纳米颗粒进行改性。 . 通过傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜、火焰原子吸收光谱和循环伏安法对合成的材料进行了表征。结果表明，合成材料的尺寸对于金纳米颗粒为 26 nm，对于 Mg-AlnHT 和 Ni-AlnHT 分别为 38.92 nm 和 8.44 nm。粘土用于改性石墨电极（GE）的表面；在水滑石结构中包含 Au 纳米颗粒改善了酶-电极的电通信。₂ O ₂通过差分脉冲伏安法在 HRP/Mg-AlnHT/GE、HRP/Ni-AlnHT/GE、HRP-AuNps/Mg-AlnHT/GE 和 HRP-AuNps/Ni-AlnHT/GE 修饰电极上测定，并且0.012μmolL的各自的检测限^-1，0.014μmolL ^-1，0.009μmolL ^-1，和0.010μmolL ^{- 1}得到。