Bacillus cereus is a concern for the dairy industry due to the biofilm and spore formation during the manufacture of dairy products, causing contamination in the final products. Biofilm spores of all tested isolates showed a significantly higher (P < 0.05) or statistically comparable D_90° value than their planktonic spores. The dipicolinic acid (DPA) content was measured and suggested not responsible for the differences in D_90° values observed. The intracellular structure of spores was observed under transmission electron microscopy, showing a clearly defined circular coat for biofilm spores while an irregular-shaped coat was observed for planktonic spores. This is the first manuscript considering factors including biofilm formation, DPA content and spore structure in milk. This suggests more severe heat treatments are needed to control B. cereus spores from biofilms in the dairy industry compared with spores from planktonic cultures without the presence of milk.

蜡状芽孢杆菌是乳制品行业关注的问题，因为在乳制品​​制造过程中会形成生物膜和孢子，导致最终产品受到污染。所有测试分离株的生物膜孢子都显示出比它们的浮游孢子显着更高（P  < 0.05）或统计学上可比的 D _90°值。对吡啶二羧酸 (DPA) 含量进行了测量，建议不对 D _90°的差异负责观察到的值。在透射电子显微镜下观察孢子的胞内结构，生物膜孢子显示出清晰的圆形外壳，而浮游孢子则观察到不规则形状的外壳。这是第一篇考虑牛奶中生物膜形成、DPA 含量和孢子结构等因素的手稿。这表明，与不存在牛奶的浮游培养物中的孢子相比，需要更严格的热处理来控制乳制品行业生物膜中的蜡样芽孢杆菌孢子。