To illustrate the influence of N6-methyladenosine long non-coding RNAs and immune cell infiltration in gastric cancer. We downloaded workflow-type data and clinical data from The Cancer Genome Atlas project. The relationship of lncRNA and m6A was identified. Kyoto Encyclopedia of Genes and Genomes gene expression enrichment analysis was performed. Lasso regression was utilized to construct a prognostic model. Survival analysis to explore the relationship between m6A lncRNA and clinical survival data. Differential analysis of the tumor microenvironment and immune correlation analysis to determine immune cell infiltration levels and their correlation with clinical prognosis. Co-expression analysis indicated that lncRNA expression was associated closely with m6A. m6A-lncRNAs were partially highly expressed in tumor tissue and could be used in a prognostic model to predict GC prognosis, independent of other clinical characteristics. “ADIPPOCYTOKINE SIGNALING PATHWAY” was most significantly enriched according to GSEA. ACBD3-AS1 was overexpressed in tumor tissue. Naïve B cell, Plasma cells, resting CD4 memory T cell were highly infiltrated tissues in cluster 2, while Macrophages M2, resting Mast cells, Monocytes, regulates T cells were lowly in cluster 1. All related scores were higher in cluster 2, indicating a lower purity of tumor cells and higher density of immune-related cells in the tumor microenvironment. m6A lncRNA is closely related to the occurrence and progression of GC. The corresponding prognostic model can be utilized to evaluate the prognosis of GC. m6A lncRNA and related immune cell infiltration in the tumor microenvironment can provide novel therapeutic targets for further research.

中文翻译：

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N6-甲基腺苷相关长链非编码RNA与胃癌肿瘤微环境中的免疫细胞浸润

说明 N6-甲基腺苷长链非编码 RNA 和免疫细胞浸润对胃癌的影响。我们从癌症基因组图谱项目下载了工作流程类型的数据和临床数据。鉴定了lncRNA和m6A的关系。进行了京都基因和基因组百科全书基因表达富集分析。套索回归用于构建预后模型。生存分析以探索 m6A lncRNA 与临床生存数据之间的关系。肿瘤微环境差异分析和免疫相关分析，以确定免疫细胞浸润水平及其与临床预后的相关性。共表达分析表明lncRNA表达与m6A密切相关。m6A-lncRNA 在肿瘤组织中部分高表达，可用于预后模型以预测 GC 预后，独立于其他临床特征。根据 GSEA，“ADIPPOCYTOKINE SIGNALING PATHWAY”最显着丰富。ACBD3-AS1 在肿瘤组织中过度表达。幼稚 B 细胞、浆细胞、静息 CD4 记忆 T 细胞在簇 2 中是高度浸润的组织，而巨噬细胞 M2、静息肥大细胞、单核细胞在簇 1 中调节 T 细胞的程度较低。所有相关分数在簇 2 中都较高，表明肿瘤微环境中肿瘤细胞纯度较低，免疫相关细胞密度较高。m6A lncRNA 与 GC 的发生发展密切相关。相应的预后模型可用于评估 GC 的预后。