ABSTRACT

In this study, amorphous hydroxyapatite (am-HAp) incorporated compressible and nanofibrous polyglycolic acid (PGA-HAp) matrices were fabricated by three-dimensional (3-D) electrospinning. The synthesised am-HAp particles were in the size of 260 ± 45 nm. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses confirmed the chemical and phase structure of the am-HAp particles. Continuous PGA-HAp nanofibers with the mean diameter of 367 ± 70 nm were successfully produced and am-HAp particles were well integrated into the nanofibers. Moreover, XRD and FTIR analyses verified the presence of am-HAp in the PGA nanofibers. The incorporation of the am-HAp to the nanofibers increased the maximum degradation temperature of PGA matrices from 340°C to 362°C. Mechanical analyses confirmed the elasticity of the 3-D PGA matrices. In vitro cell culture studies verified the biocompatibility of the nanofibrous matrices. Hence, 3-D nanofibrous PGA-HAp matrices may be a good alternative to ceramic bone substitutes due to their flexibility and physicochemical properties.

摘要

在这项研究中，无定形羟基磷灰石 (am-HAp) 掺入了可压缩和纳米纤维聚乙醇酸 (PGA-HAp) 基质，通过三维 (3-D) 静电纺丝制造。合成的 am-HAp 颗粒大小为 260 ± 45 nm。X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FTIR) 分析证实了 am-HAp 颗粒的化学和相结构。成功生产了平均直径为 367 ± 70 nm 的连续 PGA-HAp 纳米纤维，并且 am-HAp 颗粒很好地整合到纳米纤维中。此外，XRD 和 FTIR 分析证实了 PGA 纳米纤维中存在 am-HAp。am-HAp 与纳米纤维的结合使 PGA 基质的最大降解温度从 340°C 增加到 362°C。机械分析证实了 3-D PGA 矩阵的弹性。体外细胞培养研究证实了纳米纤维基质的生物相容性。因此，3-D 纳米纤维 PGA-HAp 基质由于其柔韧性和物理化学特性可能是陶瓷骨替代品的良好替代品。