Enterovirus D68 is an emerging respiratory disease pathogen causing multiple outbreaks worldwide. Enterovirus D68 strain US/KY/14-18953 was adapted to propagate in Vero cells resulting alteration of seven amino acids. The Vero cell adapted virus was inactivated with Formalin and immunized in mice. Formalin inactivated vaccine elicited high virus specific IgG antibody titer and neutralization titer. Avidity of the IgG antibodies elicited by two different doses of formalin inactivated vaccine is moderately high which got augmented by alum adjuvanted formulations. Formalin inactivated unadjuvanted vaccine elicited a balanced IgG1 type and IgG2a type antibody indicating a more balanced Th2/Th1 type immune response while alum formulated formalin inactivated antigen elicited significantly high IgG1 antibody in immunized sera and Th2 cytokines in mice splenocytes denoting Th2 type T cell immune response. Additionally, the formalin inactivated vaccine formulations has displayed excellent serum mediated invivo protective efficacy. These data suggested that formalin inactivated Enterovirus D68 is a promising vaccine candidate.

肠道病毒 D68 是一种新兴的呼吸道疾病病原体，在全球范围内引起多次暴发。肠道病毒 D68 株 US/KY/14-18953 适合在 Vero 细胞中繁殖，导致 7 个氨基酸发生改变。Vero 细胞适应病毒用福尔马林灭活并在小鼠中免疫。福尔马林灭活疫苗引起高病毒特异性 IgG 抗体滴度和中和滴度。由两种不同剂量的福尔马林灭活疫苗引发的 IgG 抗体的亲和力适中，而明矾佐剂制剂增强了这种亲和力。福尔马林灭活无佐剂疫苗引发平衡的 IgG1 型和 IgG2a 型抗体，表明 Th2/Th1 型免疫反应更平衡，而明矾配制的福尔马林灭活抗原在免疫血清中引发显着高的 IgG1 抗体和小鼠脾细胞中的 Th2 细胞因子，表明 Th2 型 T 细胞免疫反应. 此外，福尔马林灭活疫苗制剂表现出优异的血清介导体内保护功效。这些数据表明福尔马林灭活肠道病毒 D68 是一种很有前途的候选疫苗。