The concern regarding the emergence of phytopathogens strains which are resistant to conventional agrochemicals has given support to the search for alternatives on the use of chemical pesticides in agriculture. In this context, microorganisms are considered as promising sources of useful natural compounds and actinobacteria are particularly relevant since they are known to produce several bioactive metabolites. The objective of this work was to investigate the production of secondary metabolites with antifungal activity by a strain of the actinobacteria Streptomyces lunalinharesii (A54A) under axenic conditions and in co-cultivation with the phytopathogen Rhizoctonia solani. Tests to evaluate antifungal activity of the extracts indicated the presence of diffusable molecules capable of inhibiting the growth of R. solani produced by S. lunalinharesii, especially when in the presence of the fungus during fermentation. Metabolomic analyzes allowed the putative annotation of the bioactive compounds desferrioxamine E and anisomycin, in addition to the evaluation of the metabolic profile of the isolate when grown in axenic mode and in co-cultivation, while statistical analyzes enabled the comparison of such profiles and the identification of metabolites produced in greater relative quantities in the elicitation condition. Such methodologies provided the selection of unknown features with high bioactive potential for dereplication, and several metabolites of S. lunalinharesii possibly represent novel compounds.

对出现对常规农用化学品具有抗性的植物病原体菌株的担忧支持寻找在农业中使用化学杀虫剂的替代品。在这种情况下，微生物被认为是有用的天然化合物的有希望的来源，放线菌尤其重要，因为它们已知会产生几种生物活性代谢物。这项工作的目的是研究在无菌条件下和与植物病原丝核菌共培养的放线菌Streptomyces lunalinharesii (A54A)菌株产生的具有抗真菌活性的次生代谢物. 评估提取物抗真菌活性的测试表明存在能够抑制由S. lunalinharesii产生的茄属植物生长的可扩散分子，尤其是在发酵过程中存在真菌时。代谢组学分析允许对生物活性化合物去铁胺 E 和茴香霉素进行推定注释，此外还可以评估分离株在无菌模式下和共培养时的代谢特征，而统计分析可以比较这些特征和鉴定在诱导条件下以更大的相对数量产生的代谢物。这些方法提供了选择未知特征的高生物活性潜力的去复制，以及几种代谢物S. lunalinharesii可能代表新化合物。